Ubiquitin-conjugating enzyme genes in Oesophagostomum dentatum

Parasitology Research
P A CotteeRobin B Gasser

Abstract

Full-length genes representing different isoforms of the ubiquitin-conjugating enzyme UBC-2 were isolated from Oesophagostomum dentatum, cloned and sequenced. The alignment of their sequences (designated Od-ubc-2.1 to Od-ubc-2.3) revealed nucleotide variation at three positions within the predicted open reading frame of 444 bp. Substitutions were at positions 141 (A<-->G), 142 (A<-->G) and 296 (T<-->C). Both former substitutions resulted in amino acid changes from a glycine residue to an arginine residue, whereas the latter resulted in a change from isoleucine to threonine. Comparison of predicted OD-UBC-2 with UBC-2 (protein) homologues/orthologues from 12 other species representing nematodes, Drosophila melanogaster, Saccharomyces cerevisiae, mice and humans revealed identities between species varying from 77 to 100% at the amino acid level, and motifs associated with protein conformation and function were identified. While the function of a representative ubc-2 gene from O. dentatum could not be established in C. elegans, it is likely to play a key role in the catabolism of proteins and in the development of O. dentatum.

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Nov 21, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C M Pickart
Dec 31, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A L Haas, T J Siepmann
Jan 3, 2001·The Journal of Cell Biology·A GoldenD C Shakes
Jan 9, 2003·Trends in Biochemical Sciences·Rasmus Hartmann-PetersenColin Gordon
Jul 16, 2003·The Journal of Biological Chemistry·Joshua D Schnell, Linda Hicke
Apr 22, 2004·Trends in Parasitology·A Aziz Aboobaker, Mark L Blaxter
Jun 30, 2004·Cellular and Molecular Life Sciences : CMLS·S Fang, A M Weissman
Jul 1, 2004·Annals of Medicine·Pia Roos-Mattjus, Lea Sistonen
Oct 19, 2004·Genome Research·Jean-François RualMarc Vidal
Nov 9, 2004·Biochemical and Biophysical Research Communications·Michelle GudgenLynn Boyd

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Citations

May 20, 2009·Journal of Molecular Evolution·Caroline MichelleChristian R Andres
Sep 6, 2008·Parasitology·A Joachim, B Ruttkowski
Nov 15, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Martina OndrovicsAnja Joachim
Feb 11, 2015·Parasitology Research·Marcela P CostaRenata Guerra-Sá
Mar 21, 2006·Journal of the American Podiatric Medical Association·Trinity M Mereau, Timothy C Ford
May 27, 2009·Molecular Biology Reports·Hui ZhangXin-wen Bo

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