Ubiquitin-dependent chloroplast-associated protein degradation in plants.

Science
Qihua LingR Paul Jarvis

Abstract

Chloroplasts contain thousands of nucleus-encoded proteins that are imported from the cytosol by translocases in the chloroplast envelope membranes. Proteolytic regulation of the translocases is critically important, but little is known about the underlying mechanisms. We applied forward genetics and proteomics in Arabidopsis to identify factors required for chloroplast outer envelope membrane (OEM) protein degradation. We identified SP2, an Omp85-type β-barrel channel of the OEM, and CDC48, a cytosolic AAA+ (ATPase associated with diverse cellular activities) chaperone. Both proteins acted in the same pathway as the ubiquitin E3 ligase SP1, which regulates OEM translocase components. SP2 and CDC48 cooperated to bring about retrotranslocation of ubiquitinated substrates from the OEM (fulfilling conductance and motor functions, respectively), enabling degradation of the substrates by the 26S proteasome in the cytosol. Such chloroplast-associated protein degradation (CHLORAD) is vital for organellar functions and plant development.

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Citations

Nov 16, 2019·Journal of Experimental Botany·Lynn G L Richardson, Danny J Schnell
Jan 11, 2020·Biochemical Society Transactions·Simon M ThomsonR Paul Jarvis
Apr 18, 2020·Frontiers in Plant Science·Shanshan HuCheng Zhu
Apr 10, 2019·Molecular & Cellular Proteomics : MCP·Imen BouchnakNorbert Rolland
May 13, 2019·Plant Cell Reports·Najiah M SadaliR Paul Jarvis
Apr 15, 2020·Proceedings of the National Academy of Sciences of the United States of America·Chuyun GaoSuomeng Dong
May 5, 2020·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Yan WangJames Whelan
Nov 17, 2020·Frontiers in Plant Science·Hong-Yun ZengNan Yao
Nov 3, 2020·Molecular Plant·Jing-Liang SunJian-Xiang Liu
Dec 9, 2020·Plant Physiology and Biochemistry : PPB·Meshack AfitlhileDavid Hildebrand
Jul 1, 2019·Molecular & Cellular Proteomics : MCP·Imen BouchnakNorbert Rolland
Mar 8, 2021·Experimental Neurology·Xiaoyu WangJianmin Zhang
Mar 31, 2021·The Plant Journal : for Cell and Molecular Biology·Ruiqi LiuYan Xu
May 20, 2021·Nature Plants·Hsou-Min Li
Jun 3, 2021·International Journal of Molecular Sciences·Michał TraczWojciech Białek
Jun 19, 2021·Computational and Structural Biotechnology Journal·Junrui ZhangStefan T Arold
Jun 30, 2021·Journal of Experimental Botany·Mastoureh SedaghatmehrSalma Balazadeh

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