Ubiquitin-dependent degradation of cyclin B is accelerated in polyploid megakaryocytes.

The Journal of Biological Chemistry
Y ZhangK Ravid

Abstract

During the endomitotic cell cycle of megakaryocytic cell lines, the levels of cyclin B1 and the activity of cyclin B1-dependent Cdc2 kinase, although detectable, are reduced as compared with megakaryocytes undergoing a mitotic cell cycle. The levels of cyclin A, however, are comparable during both cell cycles. The expression of cyclin B1 mRNA is also equivalent in proliferating and polyploidizing cells. In the current study, we found that the rate of cyclin B1 protein degradation is enhanced in polyploidizing megakaryocytes. This finding has led us to further investigate whether the ubiquitin-proteosome pathway responsible for cyclin B degradation is accelerated in these cells. Our data indicate that polyploidizing megakaryocytic cell lines nad primary bone marrow cells treated with the megakaryocyte proliferation- and ploidy-promoting factor, the c-Mpl ligand, display increased activities of the ubiquitin-proteosome pathway, which degrades cyclin B, as compared with proliferating megakaryocytic cell lines or diploid bone marrow cells, respectively. This degradation has all the hallmarks of a ubiquitin pathway, including the dependence on ATP, the appearance of high molecular weight conjugated forms of cyclin B, and inhibition ...Continue Reading

References

Jan 10, 1991·Nature·M GlotzerM W Kirschner
Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·K RavidR D Rosenberg
Dec 1, 1991·The Journal of Cell Biology·T UsuiT Beppu
May 4, 1990·Cell·C F Lehner, P H O'Farrell
May 16, 1985·Biochemical and Biophysical Research Communications·A Hershko, H Heller
Nov 15, 1985·Biochemical and Biophysical Research Communications·Y HirataT Fujita
Feb 1, 1987·Molecular and Cellular Biology·T DoiR D Rosenberg
Nov 15, 1973·Journal of Molecular Biology·U K Laemmli, M Favre
Jul 1, 1994·Molecular Biology of the Cell·H M van der Velden, M J Lohka
Jan 1, 1994·Journal of Cell Science. Supplement·S MorenoP Nurse
Oct 7, 1994·Cell·A Ciechanover
Dec 1, 1993·The Journal of Cell Biology·K RavidR D Rosenberg
Apr 1, 1993·The Journal of Cell Biology·M PaganoG Draetta
Dec 6, 1996·Science·R W KingM W Kirschner
Jan 1, 1997·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·M RolfeM Pagano
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·F M TownsleyJ V Ruderman

❮ Previous
Next ❯

Citations

Apr 23, 2002·Journal of Cellular Biochemistry·Ying ZhangKatya Ravid
Jan 25, 2002·Journal of Cellular Physiology·Katya RavidMatthew R Jones
Jul 27, 2001·Journal of Cellular Physiology·C E CarowK Kaushansky
Jul 4, 2002·International Journal of Hematology·Itaru Matsumura, Yuzuru Kanakura
Feb 21, 2003·Biochemical and Biophysical Research Communications·Elizabeth A ConnerSnorri S Thorgeirsson
Feb 5, 1999·Gene·L M CataldoK Ravid
Mar 30, 1999·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·I Royzman, T L Orr-Weaver
Jul 23, 2003·Journal of Thrombosis and Haemostasis : JTH·B HechlerK Ravid
Jun 8, 2001·Anti-cancer Drugs· Wakita KiK Akahane
Dec 16, 2003·Molecular and Cellular Biology·Jun LuKatya Ravid
Jun 6, 2009·Cell Division·Zakir UllahMelvin L Depamphilis
Jun 13, 2001·Biochemical and Biophysical Research Communications·Y ZhangK Ravid
Feb 3, 2009·Cancer Chemotherapy and Pharmacology·Jarbas Mota SiqueiraRosa Maria Ribeiro-do-Valle
Jul 26, 2014·The Journal of Clinical Investigation·Dallas S ShiAndrew S Weyrich
Jun 12, 2013·The Journal of Cell Biology·Kellie R Machlus, Joseph E Italiano
Nov 15, 2012·International Journal of Hepatology·Géraldine GentricSéverine Celton-Morizur
Aug 15, 2009·Journal of Cellular Biochemistry·Xuerong ShiRichard K Gordon
Dec 10, 2008·Journal of Cellular Physiology·Dongchu MaJianping Cao
Jun 30, 2006·Journal of Thrombosis and Haemostasis : JTH·M Yagi, G J Roth
Sep 24, 2004·Experimental Cell Research·Carlos FernándezMiguel A Lasunción
Jan 1, 2015·Cell Discovery·Zhenghong LinDeyu Fang
Mar 19, 2014·Cellular Signalling·Chonghua LiJianping Jin
Apr 16, 2010·The Journal of Biological Chemistry·Alexia EliadesKatya Ravid
Aug 11, 2001·The Journal of Biological Chemistry·A BallesterC Calés
Sep 5, 2020·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Simon Gemble, Renata Basto
Jul 14, 2018·Toxins·Alexander P Gardner, Joseph T Barbieri

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Cell Cycle Control & Proteolysis

Key regulators of cell cycle, including cyclins, cyclin dependent kinase inhibitors, DNA replication factors, are controlled by proteolysis. Discover the latest research on cell cycle control and proteolysis.