Ubiquitin-dependent regulation of COPII coat size and function.

Nature
Lingyan JinMichael Rape

Abstract

Packaging of proteins from the endoplasmic reticulum into COPII vesicles is essential for secretion. In cells, most COPII vesicles are approximately 60-80 nm in diameter, yet some must increase their size to accommodate 300-400 nm procollagen fibres or chylomicrons. Impaired COPII function results in collagen deposition defects, cranio-lenticulo-sutural dysplasia, or chylomicron retention disease, but mechanisms to enlarge COPII coats have remained elusive. Here, we identified the ubiquitin ligase CUL3-KLHL12 as a regulator of COPII coat formation. CUL3-KLHL12 catalyses the monoubiquitylation of the COPII-component SEC31 and drives the assembly of large COPII coats. As a result, ubiquitylation by CUL3-KLHL12 is essential for collagen export, yet less important for the transport of small cargo. We conclude that monoubiquitylation controls the size and function of a vesicle coat.

References

Sep 29, 1999·Genes & Development·J D SingerJ M Roberts
Jun 25, 2005·Current Opinion in Cell Biology·J Christopher Fromme, Randy Schekman
Aug 20, 2005·Immunology·Kazuo UchidaKen-Ichi Kozaki
Jul 3, 2007·Cell·Stephan FathJonathan Goldberg
Nov 6, 2007·Developmental Cell·J Christopher FrommeLelio Orci
Jul 17, 2008·Nature Reviews. Molecular Cell Biology·Matthias Schäfer, Sabine Werner
Aug 12, 2008·Cell·Scott M StaggWilliam E Balch
Feb 10, 2009·Nature Genetics·UNKNOWN Myocardial Infarction Genetics ConsortiumDavid Altshuler
Mar 25, 2009·Nature Reviews. Cancer·Don X NguyenJoan Massagué
Jul 4, 2009·Cancer Cell·Xiang H-F ZhangJoan Massagué
Jul 30, 2009·Journal of Cell Science·Helen HughesDavid J Stephens
Oct 14, 2009·Proceedings of the National Academy of Sciences of the United States of America·Adam WilliamsonMichael Rape
Nov 12, 2009·Nature Reviews. Molecular Cell Biology·Patrick T CaswellJim C Norman
Dec 22, 2010·Journal of Cell Science·Devon Jensen, Randy Schekman
Mar 19, 2011·Cell·Richard A Young
Mar 23, 2011·Cold Spring Harbor Perspectives in Biology·Sara A WickströmReinhard Fässler
Apr 29, 2011·Molecular Biology of the Cell·Kota SaitoToshiaki Katada
May 17, 2011·Annual Review of Cell and Developmental Biology·Birgit Leitinger
May 25, 2011·The Journal of Cell Biology·Deanna G WilsonMark J Solloway
Sep 13, 2011·Molecular Cell·Woong KimSteven P Gygi
Oct 4, 2011·Cell·Michael J EmanueleStephen J Elledge

❮ Previous
Next ❯

Citations

Jun 15, 2013·Histochemistry and Cell Biology·Graham Warren
Jul 3, 2013·Histochemistry and Cell Biology·Emma Martínez-AlonsoJosé A Martínez-Menárguez
Jul 4, 2013·Histochemistry and Cell Biology·Carolyn E Machamer
Dec 18, 2013·The International Journal of Biochemistry & Cell Biology·Gokhan UnluEla W Knapik
Oct 1, 2013·Trends in Cell Biology·Rossella VendittiMaria Antonietta De Matteis
Mar 19, 2014·Trends in Cell Biology·Albert Lu, Suzanne R Pfeffer
Sep 24, 2013·Trends in Biochemical Sciences·Jure DergancAlenka Copič
Mar 5, 2013·Nature Cell Biology·Jochen BeckIzabela Sumara
Apr 4, 2013·Nature Cell Biology·Colleen A McGourty, Michael Rape
May 24, 2013·Nature Reviews. Molecular Cell Biology·Federica Brandizzi, Charles Barlowe
Dec 25, 2012·Nature Structural & Molecular Biology·Alex J NobleScott M Stagg
Sep 17, 2013·Nature Structural & Molecular Biology·Robert L JudsonRobert Blelloch
Jan 9, 2013·The Journal of Biological Chemistry·Samata TiwariShadab A Siddiqi
Jan 26, 2013·The Journal of Biological Chemistry·Peter CanningAlex N Bullock
Jun 26, 2012·Molecular & Cellular Proteomics : MCP·Daisy BustosDonald S Kirkpatrick
Sep 11, 2012·Molecular & Cellular Proteomics : MCP·J Wade Harper, Meng-Kwang Marcus Tan
May 25, 2013·Cell & Bioscience·Harish N RamanathanYihong Ye
Jan 26, 2013·PloS One·Mayuko KoreishiAyano Satoh
Apr 11, 2013·PloS One·Alan X Ji, Gilbert G Privé
Sep 27, 2013·PloS One·Jonas M la CourRandy Schekman
Feb 12, 2013·Genetics·Charles K Barlowe, Elizabeth A Miller
Mar 22, 2014·Molecular Biology of the Cell·Thomas D BairdRonald C Wek
Apr 15, 2014·Nature Communications·Kazuo KurokawaAkihiko Nakano
Sep 10, 2014·The Journal of Cell Biology·Kota SaitoToshiaki Katada
Jun 25, 2014·The Journal of Clinical Investigation·Ankit GargEric N Olson
Sep 26, 2013·ELife·Giulia ZanettiJohn A G Briggs
Dec 26, 2012·The Journal of Cell Biology·Julia von BlumeVivek Malhotra
Oct 1, 2015·Annual Review of Cell and Developmental Biology·Vivek Malhotra, Patrik Erlmann
Jun 18, 2015·Cellular and Molecular Life Sciences : CMLS·Kota Saito, Toshiaki Katada
Jan 24, 2014·Biochemical Society Transactions·Peter Canning, Alex N Bullock
Jan 27, 2015·Biochemical Society Transactions·Vivek MalhotraCristina Nogueira
Jun 7, 2013·Cellular Logistics·Michaela Hubner, Matthias Peter
May 4, 2016·The Journal of Cell Biology·António J M SantosVivek Malhotra
May 14, 2016·Molecular Biology of the Cell·Tomoya TanabeToshiaki Katada
Jun 15, 2016·Annual Review of Nutrition·Julian StevensonJames A Olzmann
Aug 24, 2016·Proceedings of the National Academy of Sciences of the United States of America·Wenfu Ma, Jonathan Goldberg
Dec 3, 2016·Nucleic Acids Research·Yu-An KungShin-Ru Shih
May 28, 2016·Nature Cell Biology·Richard Yau, Michael Rape
Aug 20, 2014·The Journal of Membrane Biology·Xiaowen ZhengJiuxiang Lin
Apr 5, 2017·Annual Review of Biochemistry·Ning Zheng, Nitzan Shabek
May 10, 2018·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Michael G HannaAnjon Audhya

❮ Previous
Next ❯

Methods Mentioned

BETA
ubiquitination
confocal microscopy
affinity purification
pulldown
electron microscopy
transfection
light microscopy
PCR
scraping
transfections

Software Mentioned

ImageJ
LSM image Browser
Zen10
- Blast
LSM browser
Primer
Imaris

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.