Abstract
Ubiquitin gene expression following transient forebrain ischemia in the rat was analyzed by three probes which were specific for UbC, UbB and UbS30 mRNA. According to the in situ hybridization studies, each type of ubiquitin gene expression decreased at 30 min of reperfusion following 20 min of forebrain ischemia, thereafter increased, and then reached a peak at 4-6 h, both in the cortex and hippocampus. These changes returned to the control level after 24-48 h of recirculation. Among the three ubiquitin transcripts, changes in UbC expression were more marked in the hippocampus, and persistent expression of UbC transcripts in the CA1 and CA3 regions was observed at 24 h of reperfusion. With dot-blot analysis, significant increases in the UbC transcripts were noted at 4 h of reperfusion in the hippocampus, and at 6 h in the cortex following 20 min of ischemia. These results suggest that changes in UbC expression might be a good indicator of ischemic stress.
References
May 1, 1979·Stroke; a Journal of Cerebral Circulation·W A Pulsinelli, J B Brierley
Sep 16, 1992·Biochemical and Biophysical Research Communications·K KasJ Merregaert
Jun 1, 1992·Neuroreport·T HayashiM Matsuda
Feb 1, 1992·International Journal of Radiation Biology·M Nenoi
Feb 11, 1990·Journal of Neurochemistry·T S NowakM J Schlesinger
Mar 11, 1991·Nucleic Acids Research·R T Baker, P G Board
May 1, 1991·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·T S Nowak
Feb 1, 1985·Journal of Neurochemistry·G A DienelS J Rosenfeld
May 1, 1985·Molecular and Cellular Biology·U Bond, M J Schlesinger
Dec 1, 1986·Molecular and Cellular Biology·U Bond, M J Schlesinger
Jan 26, 1987·Nucleic Acids Research·R T Baker, P G Board
Oct 7, 1994·Cell·A Ciechanover
Jun 21, 1994·Biochimica Et Biophysica Acta·T HayashiM Matsuda
Oct 15, 1993·Journal of Neuroscience Research·F M LongoF R Sharp
May 28, 1993·Annals of the New York Academy of Sciences·C G CadayS P Finklestein
Aug 20, 1993·Brain Research·T HayashiM Matsuda
Citations
Mar 18, 2006·Apoptosis : an International Journal on Programmed Cell Death·H ShenQ Y Liu
Mar 18, 2009·Cellular and Molecular Neurobiology·Eva MechírováJozef Burda
Feb 19, 2004·Brain Research. Molecular Brain Research·Toshihito NagataSatoshi Asai
May 9, 2006·Antioxidants & Redox Signaling·H Fai PoonD Allan Butterfield
May 5, 2004·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Joan Smith SonnebornPaul Thomas
Mar 24, 1997·Neuroreport·M NogaJ Tanaka
Apr 16, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·S J VannucciP W Beesley
Apr 21, 2010·PloS One·Márcio S BaptistaCarlos B Duarte
Aug 5, 2010·Journal of Cellular Physiology·Meng Shyan ChoyNam Sang Cheung
Aug 27, 2017·Translational Stroke Research·Karin Hochrainer
Nov 20, 2018·Journal of Neurochemistry·Jada H VadenScott M Wilson
Feb 8, 2006·Academic Emergency Medicine : Official Journal of the Society for Academic Emergency Medicine·Peter R OeltgenDonald B Witzke