Ubiquitin ligase Rad18Sc localizes to the XY body and to other chromosomal regions that are unpaired and transcriptionally silenced during male meiotic prophase

Journal of Cell Science
Roald van der LaanWilly M Baarends

Abstract

In replicative damage bypass (RDB) in yeast, the ubiquitin-conjugating enzyme RAD6 interacts with the ubiquitin ligase RAD18. In the mouse, these enzymes are represented by two homologs of RAD6, HR6a and HR6b, and one homolog of RAD18, Rad18Sc. Expression of these genes and the encoded proteins is ubiquitous, but there is relatively high expression in the testis. We have studied the subcellular localization by immunostaining Rad18Sc and other RDB proteins in mouse primary spermatocytes passing through meiotic prophase in spermatogenesis. The highest Rad18Sc protein level is found at pachytene and diplotene, and the protein localizes mainly to the XY body, a subnuclear region that contains the transcriptionally inactivated X and Y chromosomes. In spermatocytes that carry translocations for chromosomes 1 and 13, Rad18Sc protein concentrates on translocation bivalents that are not fully synapsed. The partly synapsed bivalents are often localized in the vicinity of the XY body, and show a very low level of RNA polymerase II, indicating that the chromatin is in a silent configuration similar to transcriptional silencing of the XY body. Thus, Rad18Sc localizes to unsynapsed and silenced chromosome segments during the male meiotic pro...Continue Reading

References

Jan 1, 1991·Methods in Cell Biology·C Heyting, A J Dietrich
Jul 8, 1988·Journal of Theoretical Biology·E Jablonka, M J Lamb
Nov 26, 1984·Biochimica Et Biophysica Acta·J A GrootegoedH J Van der Molen
Aug 1, 1995·Developmental Biology·P J HendriksenJ A Grootegoed
Apr 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C Lawrence
Jun 1, 1996·Current Opinion in Cell Biology·C Heyting
Oct 1, 1996·Trends in Genetics : TIG·J Forejt
Feb 1, 1997·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·A H PetersP de Boer
Aug 1, 1997·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·A H PetersP de Boer
Jan 4, 1998·Biology of Reproduction·D KamelJ E Syväoja
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·S BroomfieldW Xiao
Mar 9, 1999·Developmental Biology·W M BaarendsJ A Grootegoed
Jun 26, 1999·Chromosoma·P B MoensA Wynshaw-Boris
Sep 15, 1999·Genes & Development·R Woodgate
Jan 22, 2000·Science·K RobzykM A Osley
Feb 9, 2000·Trends in Biochemical Sciences·K Baynton, R P Fuchs
Jul 8, 2000·Proceedings of the National Academy of Sciences of the United States of America·S TateishiM Yamaizumi
Jul 25, 2000·Nucleic Acids Research·H XinZ Wang
Mar 10, 2001·Nature Genetics·S K MahadevaiahP S Burgoyne
Jun 19, 2002·The Journal of Biological Chemistry·Jim DoverAli Shilatifard
Jan 2, 2003·Molecular and Cellular Biology·Satoshi TateishiMasaru Yamaizumi
Apr 12, 2003·Developmental Cell·Oscar Fernandez-CapetilloAndré Nussenzweig

❮ Previous
Next ❯

Citations

Mar 3, 2012·Cellular and Molecular Life Sciences : CMLS·Yosuke IchijimaSatoshi H Namekawa
Aug 4, 2007·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·William G Kelly, Rodolfo Aramayo
Jul 3, 2013·Nature Communications·Lin-Yu LuXiaochun Yu
Feb 4, 2009·Nature Reviews. Genetics·Paul S BurgoyneJames M A Turner
Jan 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jee Young AnYong Tae Kwon
Aug 28, 2007·Nucleic Acids Research·Valerie NotenboomTitia K Sixma
Aug 25, 2007·Genome Research·David HomolkaJiri Forejt
Jan 20, 2005·Molecular and Cellular Biology·Willy M BaarendsJ Anton Grootegoed
Aug 3, 2011·Journal of Cell Science·Akiko InagakiWilly M Baarends
Feb 23, 2012·PloS One·Marieke de VriesPeter de Boer
May 26, 2005·Molecular Reproduction and Development·Laura L Tres
Dec 21, 2006·Journal of Cellular Biochemistry·Danuta GaletzkaThomas Haaf
Feb 11, 2010·International Journal of Cell Biology·Isabelle GillotPascal Lopez
Dec 14, 2004·Current Biology : CB·James M A TurnerChu-Xia Deng
Dec 11, 2008·Mechanisms of Development·Jinghua SunSatoshi Tateishi
Jun 12, 2010·Genetika·E A Vas'kovaS M Zakinian
Aug 4, 2016·Oncotarget·Curtis D HodgeJ N Mark Glover
Jul 3, 2016·Genetics·Andrew P MorganFernando Pardo-Manuel de Villena

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.