Ubiquitin proteasome system and the atypical kinase PfPK7 are involved in melatonin signaling in Plasmodium falciparum.

Journal of Pineal Research
Fernanda C KoyamaCelia R S Garcia

Abstract

We previously reported that melatonin modulates the Plasmodium falciparum erythrocytic cycle by increasing schizont stage population as well as diminishing ring stage population. In addition, the importance of calcium and cAMP in melatonin signaling pathway in P. falciparum was also demonstrated. Nevertheless, the molecular effectors of the indoleamine signaling pathway remain elusive. We now demonstrate by real-time PCR that melatonin treatment up-regulates genes related to ubiquitin/proteasome system (UPS) components and that luzindole, a melatonin receptor antagonist, inhibits UPS transcription modulation. We also show that protein kinase PfPK7, a P. falciparum orphan kinase, plays a crucial role in the melatonin transduction pathway, since following melatonin treatment of P. falciparum parasites where pfpk7 gene is disrupted (pfpk7(-) parasites) (i) the ratio of asexual stages remain unchanged, (ii) the increase in cytoplasmatic calcium in response to melatonin was strongly diminished and (iii) up-regulation of UPS genes did not occur. The wild-type melatonin-induced alterations in cell cycle features, calcium rise and UPS gene transcription were restored by re-introduction of a functional copy of the pfpk7 gene in the pfp...Continue Reading

References

Aug 20, 1976·Science·W Trager, J B Jensen
Feb 1, 1995·Trends in Pharmacological Sciences·M L Dubocovich
Jan 1, 1993·Life Sciences·G Benítez-KingF Antón-Tay
Oct 6, 1998·Annual Review of Biochemistry·A Hershko, A Ciechanover
Dec 19, 1998·The EMBO Journal·A Ciechanover
Sep 25, 1999·Journal of Pineal Research·M MacíasD Acuña-Castroviejo
Jun 14, 2001·International Journal for Parasitology·M L Caldas, M Wasserman
Oct 24, 2001·Journal of Biological Rhythms·C R GarciaL Madeira
Jan 18, 2003·Cell Calcium·Fernando P VarottiCélia R S Garcia
Oct 24, 2003·Cell Calcium·Jason I E BruceDavid I Yule
Oct 25, 2003·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·C T HottaC R S Garcia
Apr 24, 2004·Trends in Parasitology·David A Baker, John M Kelly
Sep 11, 2004·Biochemical and Biophysical Research Communications·Marcos L Gazarini, Célia R S Garcia
Jun 6, 2006·Molecular Biology and Evolution·Kisaburo Nagamune, L David Sibley
Mar 14, 2007·Journal of Pineal Research·Alexandre BuduCélia Regina da Silva Garcia
Oct 25, 2007·BMC Clinical Pharmacology·Jennifer M ReynoldsChoukri Ben Mamoun
Jan 1, 2008·The Journal of Biological Chemistry·Ankush VaidPushkar Sharma
Jun 12, 2008·International Review of Cell and Molecular Biology·Celia R S GarciaLawrence Bannister
Apr 28, 2009·Molecular and Biochemical Parasitology·Fernanda C KoyamaCélia R S Garcia
Jun 6, 2009·The Journal of Biological Chemistry·Luc ReiningerChristian Doerig
Oct 21, 2009·Parasite : Journal De La Société Française De Parasitologie·C DoerigP Alano
Dec 23, 2009·Journal of Pineal Research·Venkataramanujan SrinivasanDaniel P Cardinali
Oct 23, 2010·Journal of Pineal Research·Marcos L GazariniCélia R S Garcia
Jan 7, 2011·Proceedings. Biological Sciences·Aidan J O'DonnellSarah E Reece
Oct 11, 2011·Molecular and Biochemical Parasitology·Piero BagnaresiCélia R S Garcia

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Citations

May 16, 2012·Microbes and Infection·Alexandre Budu, Célia R S Garcia
Jul 11, 2013·International Journal of Molecular Sciences·Wânia Rezende LimaCélia R S Garcia
Feb 18, 2021·Trends in Parasitology·Jack AdderleyChristian Doerig
Apr 15, 2021·Medicinal Research Reviews·Yash GuptaPrakasha Kempaiah
Aug 25, 2021·Current Opinion in Microbiology·Benedito M Dos SantosCélia Rs Garcia

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