Ubiquitin-protein conjugates selectively distribute during early chicken embryogenesis

Developmental Dynamics : an Official Publication of the American Association of Anatomists
A M Wunsch, A L Haas

Abstract

The major mechanism for proteolysis in eucaryotes involves an ATP-dependent pathway for which the covalent attachment of ubiquitin targets proteins for degradation. The involvement of ubiquitin conjugation in early embryonic vertebrate development was investigated by examining the amounts and localization of ubiquitin conjugates at different stages of development in the chicken using an affinity-purified antibody specific for conjugated ubiquitin. Solid phase immunochemical assays measuring whole embryo pools of free and conjugated ubiquitin demonstrated a progressive increase in conjugate pools to stage 18, followed by a decline to stage 24. In contrast, levels of free polypeptide showed a dramatic increase after stage 5, indicating a change in the dynamics of the two pools during development. Immunohistochemistry revealed that the distribution of ubiquitin adducts between stages 3 and 22 was pronounced in regions undergoing extensive cellular remodeling. Ubiquitin conjugates were detected in the primitive streak where cells ingress during gastrulation. The presence of these degradative intermediates in both neuroectodermal cells of the neural folds and subsequent neural crest cells migrating from the dorsum of the neural tube...Continue Reading

References

Jan 1, 1992·Annual Review of Biochemistry·A Hershko, A Ciechanover
Jan 1, 1992·Annual Review of Genetics·S Jentsch
Mar 1, 1992·The Journal of Experimental Zoology·I S Alvarez, G C Schoenwolf
Jan 1, 1991·Annual Review of Cell Biology·D Finley, V Chau
Jan 15, 1992·European Journal of Biochemistry·A L Goldberg
Jan 1, 1991·Annual Review of Cell Biology·R E EllisH R Horvitz
Jan 10, 1991·Nature·M GlotzerM W Kirschner
Jan 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A CiechanoverG M Brodeur
May 1, 1991·Developmental Biology·P A HulleyS H Kidson
Dec 1, 1990·The Journal of Cell Biology·U KleinP M Kloetzel
Jul 1, 1989·Archives of Biochemistry and Biophysics·C M PickartS F Dosch
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K G MurtiV A Fried
Jun 1, 1988·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D A RileyA L Haas
Jan 1, 1974·International Review of Cytology·P Karfunkel
Jul 1, 1974·Developmental Biology·J A MacCabeJ W Saunders
Jun 1, 1982·Developmental Biology·D H Bernanke, R R Markwald
Aug 1, 1962·Developmental Biology·J W SAUNDERS, M T GASSELING
Jan 1, 1951·Journal of Morphology·V HAMBURGER, H L HAMILTON

❮ Previous
Next ❯

Citations

May 8, 2001·Genetics in Medicine : Official Journal of the American College of Medical Genetics·H P De Decker, J B Lawrenson
Mar 3, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Takashi MikawaDavid E Reese
Mar 18, 1999·Nature Genetics·A Baldini
Jul 12, 2002·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kristine A KellyTakashi Mikawa
Feb 1, 1997·Human Molecular Genetics·A PizzutiB Dallapiccola
Jan 14, 2003·The Journal of Biological Chemistry·Thomas J SiepmannArthur L Haas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
I WefesD Finley
Journal of Medical Genetics
M A O'Reilly, C M Hall
In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association
J J BergerA Taylor
© 2021 Meta ULC. All rights reserved