Ubiquitination is required for the retro-translocation of a short-lived luminal endoplasmic reticulum glycoprotein to the cytosol for degradation by the proteasome.

The Journal of Biological Chemistry
M de VirgilioN E Ivessa

Abstract

In the endoplasmic reticulum (ER), an efficient "quality control system" operates to ensure that mutated and incorrectly folded proteins are selectively degraded. We are studying ER-associated degradation using a truncated variant of the rough ER-specific type I transmembrane glycoprotein, ribophorin I. The truncated polypeptide (RI332) consists of only the 332 amino-terminal amino acids of the protein corresponding to most of its luminal domain and, in contrast to the long-lived endogenous ribophorin I, is rapidly degraded. Here we show that the ubiquitin-proteasome pathway is involved in the destruction of the truncated ribophorin I. Thus, when RI332 that itself appears to be a substrate for ubiquitination was expressed in a mutant hamster cell line harboring a temperature-sensitive mutation in the ubiquitin-activating enzyme E1 affecting ubiquitin-dependent proteolysis, the protein is dramatically stabilized at the restrictive temperature. Moreover, inhibitors of proteasome function effectively block the degradation of RI332. Cell fractionation experiments indicate that RI332 accumulates in the cytosol when degradation is prevented by proteasome inhibitors but remains associated with the lumen of the ER under ubiquitination-...Continue Reading

References

Aug 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A J RivettE Knecht
Aug 1, 1991·Current Opinion in Cell Biology·J S Bonifacino, J Lippincott-Schwartz
Aug 24, 1990·Cell·R D Klausner, R Sitia
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B J JasminJ P Changeux
Jan 1, 1989·Annual Review of Cell Biology·S M Hurtley, A Helenius
Nov 1, 1987·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J F Dice
Jan 1, 1988·Annual Review of Cell Biology·J K Rose, R W Doms
Apr 4, 1986·Science·M S Brown, J L Goldstein
Jun 15, 1985·The Biochemical Journal·N Lambert, R B Freedman
Sep 1, 1984·The Journal of Cell Biology·M G RosenfeldG Kreibich
Aug 1, 1995·Current Opinion in Cell Biology·C Hammond, A Helenius
Oct 6, 1995·Cell·C L WardR R Kopito
Nov 3, 1995·The Journal of Biological Chemistry·J G Laing, E C Beyer
Oct 7, 1994·Cell·A Ciechanover
Mar 1, 1994·Trends in Biochemical Sciences·J J BergeronD B Williams
Jul 1, 1993·Molecular Biology of the Cell·A A McCracken, K B Kruse
Feb 1, 1996·The Journal of Cell Biology·A A McCracken, J L Brodsky
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·E D WernerA A McCracken
Jan 1, 1996·Annual Review of Genetics·M Hochstrasser

❮ Previous
Next ❯

Citations

Dec 9, 2003·Trends in Biochemical Sciences·Wayne I Lencer, Billy Tsai
Jun 17, 2000·Trends in Cell Biology·C Hirsch, H L Ploegh
Jan 15, 2000·Molecular Cell·M Zhou, R Schekman
Jun 25, 2004·Nature·Brendan N Lilley, Hidde L Ploegh
Aug 2, 2005·Nature Cell Biology·Birgit MeusserThomas Sommer
Dec 3, 2013·Nature Cell Biology·Martin MehnertErnst Jarosch
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jan RiemerLars Ellgaard
Jun 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Matthias ZehnerSven Burgdorf
Sep 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·Takashi ImaiHeiichiro Udono
Jun 16, 2001·Molecular Biology of the Cell·S Kamhi-NesherG Z Lederkremer
Aug 2, 2005·Molecular Biology of the Cell·Samiksha KatiyarWilliam J Lennarz
Mar 21, 2001·EMBO Reports·R R Kopito, R Sitia
Feb 16, 2002·The EMBO Journal·Tiziana AnelliRoberto Sitia
Jan 26, 2008·Glycobiology·Isabelle Chantret, Stuart E H Moore
Aug 17, 2006·Journal of Virology·Brendan N LilleyThomas L Benjamin
Jan 5, 2002·Molecular and Cellular Biology·Efrat RabinovichShoshana Bar-Nun
Apr 7, 2011·PLoS Biology·Robert ErnstHidde L Ploegh
Mar 7, 2014·PloS One·Jasper H L ClaessenHidde L Ploegh
Oct 30, 1999·Biological Chemistry·M H SkowronekI G Haas
Jan 9, 2010·International Journal of Molecular Sciences·Hideya AndoVincent J Hearing
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·C M StoryH L Ploegh
Dec 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·A Di ColaL M Roberts
Jul 27, 2006·Proceedings of the National Academy of Sciences of the United States of America·Dennis FliermanVincent Chau
Sep 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·H ParkW J Lennarz
May 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Michael A Rycyzyn, Charles V Clevenger
Nov 3, 2011·Protoplasma·Pascal St Pierre, Ivan R Nabi
Jul 11, 2001·Biochemical and Biophysical Research Communications·C A MosseV H Engelhard
Mar 22, 2000·The Journal of Cell Biology·A SchmitzV Herzog
Jun 13, 2012·The Journal of Cell Biology·Eric M RubensteinMark Hochstrasser
Oct 18, 2006·The Journal of Cell Biology·Britta MuellerHidde L Ploegh
Jun 1, 2000·The Journal of Cell Biology·T SuzukiW J Lennarz
Jan 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Chengcheng HuangTadashi Suzuki

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.