Ubiquitous Chromatin-opening Elements (UCOEs): Applications in biomanufacturing and gene therapy

Biotechnology Advances
Jonathan J NevilleMichael Nicholas Antoniou

Abstract

Ubiquitous Chromatin-opening Elements (UCOEs) are defined by their ability to consistently confer stable, site of integration-independent transgene expression that is proportional to copy number, including from within regions of heterochromatin such as centromeres. UCOEs structurally consist of methylation-free CpG islands encompassing single or dual divergently-transcribed housekeeping gene promoters. Since their discovery in 1999, UCOEs and their sub-fragments have found applications in areas of biotechnology requiring stable, reproducible, and high levels of gene expression. This review recounts the discovery of UCOEs and examines their current and future applications in protein therapeutic biomanufacturing and gene therapy.

Citations

Feb 6, 2018·Applied Biochemistry and Biotechnology·Seyedeh Hoda JazayeriMohammad Reza Khorramizadeh
Sep 1, 2017·Human Gene Therapy·Saskia KohlscheenUte Modlich
Sep 29, 2018·Current Protocols in Protein Science·Molly HunterMark Fox
Dec 6, 2018·Biotechnology & Genetic Engineering Reviews·Suleiman Yusuf AlhajiSyahril Abdullah
Oct 12, 2019·Science·Arash FarhadiMikhail G Shapiro
Jul 18, 2017·Frontiers in Plant Science·Ganapathy SivakumarGregory C Phillips
Jul 7, 2019·Biotechnology Advances·Kritika GuptaPrajakta Dandekar
Mar 16, 2021·Molecular Therapy. Methods & Clinical Development·Brenda J SeymourDavid J Rawlings

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