Abstract
The 3' terminal region (3'-X tail) of hepatitis C virus (HCV) genomic RNA forms a stable stem-loop structure. The 3'-X tail consists of 98 nucleotides (nt) that are highly conserved among the HCV strains and supposed to function as a cis-acting region for replication of negative strand RNA and/or viral encapsidation. In the present study, by UV cross-linking assay we found two kinds of cellular proteins of approximately 87 and 130 kDa, which specifically bind to the full-length 3'-X tail (nt 1 to 98), but not the 3'- or 5'-truncated 3'-X tail, consisting of nt 1 to 50 or nt 51 to 98, respectively. These proteins were detected in human cell lines such as hepatic tumor cell lines and a T-lymphocyte cell line and also in a human embryonic lung fibroblast cell strain. In addition, human hepatocellular carcinoma tissues expressed these proteins regardless of infection or uninfection of HCV. Furthermore, these proteins were also detected in normal human tissues derived from the lung, heart, kidney, stomach, intestine, and colon. Thus, these cellular proteins, which are ubiquitously present in human tissues, might be involved in viral replication and/or encapsidation.
References
Jan 1, 1979·Annual Review of Biochemistry·T Blumenthal, G G Carmichael
Jul 25, 1992·Nucleic Acids Research·A GhettiG Dreyfuss
Nov 7, 1991·The New England Journal of Medicine·R D AachG J Nemo
Oct 12, 1991·Lancet·M OmataM Ohto
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·Q L ChooP J Barr
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·N KatoK Shimotohno
Oct 19, 1990·Cell·R J Hayes, K W Buck
Aug 11, 1989·Nucleic Acids Research·E SchreiberW Schaffner
Sep 12, 1986·Cell·M M Konarska, P A Sharp
Jul 30, 1986·Virology·T TakegamiK Yasui
Aug 23, 1985·Science·C M RiceJ H Strauss
Aug 15, 1970·Nature·U K Laemmli
Oct 13, 1995·Biochemical and Biophysical Research Communications·T TanakaK Shimotohno
Oct 1, 1995·Journal of Virology·N Ali, A Siddiqui
Mar 1, 1993·Journal of Virology·A GrakouiC M Rice
Apr 20, 1995·Virology·J H YenM F Chang
Jan 10, 1995·Virology·A K PattnaikG W Wertz
Jan 26, 1995·Biochemical and Biophysical Research Communications·N KatoK Shimotohno
Jan 1, 1995·Journal of Virology·B J YooJ H Han
Dec 1, 1993·Journal of Virology·T Furuya, M M Lai
Feb 1, 1993·Journal of Virology·R LeopardiA A Salmi
May 1, 1996·Journal of Virology·T TanakaK Shimotohno
Jun 1, 1996·Journal of Virology·A A KolykhalovC M Rice
Feb 1, 1996·Journal of Medical Virology·S TsuboiM Namba
Jul 25, 1997·Science·A A KolykhalovC M Rice
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·M YanagiJ Bukh
Sep 1, 1997·Journal of Virology·J L Blackwell, M A Brinton
Sep 1, 1997·Journal of Virology·K TsuchiharaK Shimotohno
Oct 6, 1997·Journal of Virology·K J Blight, C M Rice
Citations
Dec 11, 1999·Proceedings of the Association of American Physicians·D W Bradley
Mar 17, 2001·Microbial & Comparative Genomics·N Kato
Feb 1, 2005·Nucleic Acids Research·Mariola Dutkiewicz, Jerzy Ciesiolka
Jan 11, 2001·Journal of Virology·T PietschmannR Bartenschlager
Jan 11, 2001·Journal of Virology·J WoodA H Patel
Sep 5, 2002·Journal of Virology·Robert M SmithGeorge Y Wu
Sep 13, 2003·Journal of Virology·Lieven J StuyverMichael J Otto
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·M YanagiJ Bukh
Dec 11, 2002·Journal of Gastroenterology and Hepatology·Stephen A Locarnini
Mar 13, 2012·Virology·Sang-Yun ChoKook-Hyung Kim
Jun 22, 2000·The Journal of General Virology·R Bartenschlager, V Lohmann