Ubiquitous presence of cellular proteins that specifically bind to the 3' terminal region of hepatitis C virus

Biochemical and Biophysical Research Communications
Y InoueM Namba

Abstract

The 3' terminal region (3'-X tail) of hepatitis C virus (HCV) genomic RNA forms a stable stem-loop structure. The 3'-X tail consists of 98 nucleotides (nt) that are highly conserved among the HCV strains and supposed to function as a cis-acting region for replication of negative strand RNA and/or viral encapsidation. In the present study, by UV cross-linking assay we found two kinds of cellular proteins of approximately 87 and 130 kDa, which specifically bind to the full-length 3'-X tail (nt 1 to 98), but not the 3'- or 5'-truncated 3'-X tail, consisting of nt 1 to 50 or nt 51 to 98, respectively. These proteins were detected in human cell lines such as hepatic tumor cell lines and a T-lymphocyte cell line and also in a human embryonic lung fibroblast cell strain. In addition, human hepatocellular carcinoma tissues expressed these proteins regardless of infection or uninfection of HCV. Furthermore, these proteins were also detected in normal human tissues derived from the lung, heart, kidney, stomach, intestine, and colon. Thus, these cellular proteins, which are ubiquitously present in human tissues, might be involved in viral replication and/or encapsidation.

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Citations

Dec 11, 1999·Proceedings of the Association of American Physicians·D W Bradley
Jan 11, 2001·Journal of Virology·T PietschmannR Bartenschlager
Jan 11, 2001·Journal of Virology·J WoodA H Patel
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·M YanagiJ Bukh
Dec 11, 2002·Journal of Gastroenterology and Hepatology·Stephen A Locarnini
Jun 22, 2000·The Journal of General Virology·R Bartenschlager, V Lohmann

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