Ubiquitylation functions in the calcium carbonate biomineralization in the extracellular matrix.

PloS One
Dong FangRongqing Zhang

Abstract

Mollusks shell formation is mediated by matrix proteins and many of these proteins have been identified and characterized. However, the mechanisms of protein control remain unknown. Here, we report the ubiquitylation of matrix proteins in the prismatic layer of the pearl oyster, Pinctada fucata. The presence of ubiquitylated proteins in the prismatic layer of the shell was detected with a combination of western blot and immunogold assays. The coupled ubiquitins were separated and identified by Edman degradation and liquid chromatography/mass spectrometry (LC/MS). Antibody injection in vivo resulted in large amounts of calcium carbonate randomly accumulating on the surface of the nacreous layer. These ubiquitylated proteins could bind to specific faces of calcite and aragonite, which are the two main mineral components of the shell. In the in vitro calcium carbonate crystallization assay, they could reduce the rate of calcium carbonate precipitation and induce the calcite formation. Furthermore, when the attached ubiquitins were removed, the functions of the EDTA-soluble matrix of the prismatic layer were changed. Their potency to inhibit precipitation of calcium carbonate was decreased and their influence on the morphology of c...Continue Reading

References

Jan 1, 1975·Journal of Biomechanics·D T Reilly, A H Burstein
Feb 28, 1992·Science·A H HeuerD H Thompson
Jan 1, 1974·Calcified Tissue Research·J D Currey, K Brear
Mar 13, 1981·Science·H A Lowenstam
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·H MiyamotoA Matsushiro
Jun 5, 1997·Nature·S SudoT Takahashi
May 21, 1998·Journal of Biomechanics·R Z Wang, S Weiner
Jan 14, 2000·Journal of Biomechanical Engineering·M T FondrkD T Davy
Jul 2, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yong ZhangRongqing Zhang
May 18, 2004·Biochemical and Biophysical Research Communications·Hirotaka InoueHiromichi Nagasawa
Jul 14, 2004·Biochemical and Biophysical Research Communications·Daiki TsukamotoKazuyoshi Endo
May 24, 2005·Biomaterials·Marthe RousseauXavier Bourrat
Jul 5, 2005·The Journal of Biological Chemistry·Frédéric MarinPeter Westbroek
Sep 16, 2005·Angewandte Chemie·Yuya Oaki, Hiroaki Imai
Apr 25, 2006·Biochemical and Biophysical Research Communications·Cen ZhangRongqing Zhang
Aug 19, 2007·Biochemical and Biophysical Research Communications·Masato YanoHiroshi Miyamoto
Feb 13, 2008·Molecular Ecology·J Cristobal VeraJames H Marden
May 16, 2008·Proceedings of the National Academy of Sciences of the United States of America·Assaf ShechterAmir Sagi
Aug 30, 2008·Journal of the Royal Society, Interface·Nan YaoNing Yang
Apr 25, 2009·Nature Reviews. Molecular Cell Biology·Ivan Dikic
Jul 3, 2009·Journal of Cell Science·Keith D Wilkinson
Aug 15, 2009·Science·Michio SuzukiHiromichi Nagasawa
Sep 29, 2009·BMC Evolutionary Biology·Tokiharu TakahashiDavid E K Ferrier
Apr 12, 2011·Chemical Communications : Chem Comm·Siddharth V Patwardhan

❮ Previous
Next ❯

Methods Mentioned

BETA
x-ray spectroscopy
dot blot
X-ray
dot-blot

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.