Ubiquitylation of the acetyltransferase MOF in Drosophila melanogaster.

PloS One
Sarah SchunterPeter B Becker

Abstract

The nuclear acetyltransferase MOF (KAT8 in mammals) is a subunit of at least two multi-component complexes involved in transcription regulation. In the context of complexes of the 'Non-Specific-Lethal' (NSL) type it controls transcription initiation of many nuclear housekeeping genes and of mitochondrial genes. While this function is conserved in metazoans, MOF has an additional, specific function in Drosophila in the context of dosage compensation. As a subunit of the male-specific-lethal dosage compensation complex (MSL-DCC) it contributes to the doubling of transcription output from the single male X chromosome by acetylating histone H4. Proper dosage compensation requires finely tuned levels of MSL-DCC and an appropriate distribution of MOF between the regulatory complexes. The amounts of DCC formed depends directly on the levels of the male-specific MSL2, which orchestrates the assembly of the DCC, including MOF recruitment. We found earlier that MSL2 is an E3 ligase that ubiquitylates most MSL proteins, including MOF, suggesting that ubiquitylation may contribute to a quality control of MOF's overall levels and folding state as well as its partitioning between the complex entities. We now used mass spectrometry to map the...Continue Reading

References

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Citations

Dec 13, 2018·Nucleic Acids Research·Christian AlbigPeter B Becker
Dec 6, 2019·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Wladyslaw A Krajewski
Feb 23, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Jasmine A Burrell, Jacqueline M Stephens
Jun 20, 2021·Nature Communications·Praveen BawankarJean-Yves Roignant

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Methods Mentioned

BETA
acetylation
acetylates
proximity ligation assay
proximity ligation
immunoprecipitation
co-immunoprecipitation
pull down
transgenic
Assay
PCR

Software Mentioned

Adobe Illustrator
CellProfiler
Andromeda
Adobe Photoshop
MaxQuant

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