UCA1 promotes cell proliferation and invasion and inhibits apoptosis through regulation of the miR129-SOX4 pathway in renal cell carcinoma

OncoTargets and Therapy
Qian LiuChaoyang Zhu

Abstract

Renal cell carcinoma (RCC) is the most common cancer in kidney malignancies. UCA1 has been identified as an oncogenic lncRNA in multiple cancers, including RCC. However, the underlying molecular mechanism of UCA1 involved in RCC progression is far from being addressed. Reverse-transcription quantitative polymerase chain reaction (RT-qPCR) assays were used to measure expressions of UCA1, miR129, and SOX4 mRNA. Western blot assays were employed to detect SOX4 protein expression. Cell proliferation, invasion, and apoptosis were assessed by CCK-8, Matrigel invasion, and annexin-fluorescein isothiocyanate (FITC) apoptosis-detection assays, respectively. The interaction between UCA1 and miR129 was demonstrated by luciferase, RNA pull-down, and RNA-immunoprecipitation (RIP) assays. Luciferase assays were also used to explore whether UCA1 was able to act as a molecular sponge of miR129 to affect the interplay of miR129 and SOX4. UCA1 expression was upregulated in RCC tissue and cells, and higher UCA1 expression was associated with advanced pathogenic status and poor prognosis of RCC patients. UCA1 knockdown suppressed proliferation and invasion and induced apoptosis in RCC cells. UCA1 inhibited miR129 expression by direct interaction i...Continue Reading

Citations

Jun 6, 2019·FEBS Letters·Wei XuanDandan Song
Feb 29, 2020·International Journal of Molecular Sciences·Satoshi KubotaTakashi Nishida
Nov 18, 2018·Cancers·Bernadette NeveIsabelle Van Seuningen
Jul 25, 2018·International Journal of Molecular Sciences·Alessandro La FerlitaCinzia Di Pietro
Jun 1, 2021·Frontiers in Cell and Developmental Biology·Clara ApicellaFrancisco Miralles

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Methods Mentioned

BETA
transfection
PCR
electrophoresis
flow cytometry
RIP
pull-down
ubiquitination

Software Mentioned

miRcode

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