UDP-Glucose 4-Epimerase and β-1,4-Galactosyltransferase from the Oyster Magallana gigas as Valuable Biocatalysts for the Production of Galactosylated Products

International Journal of Molecular Sciences
Hui-Bo SongJosef Voglmeir

Abstract

Uridine diphosphate galactose (UDP-galactose) is a valuable building block in the enzymatic synthesis of galactose-containing glycoconjugates. UDP-glucose 4-epimerase (UGE) is an enzyme which catalyzes the reversible conversion of abundantly available UDP-glucose to UDP-galactose. Herein, we described the cloning, expression, purification, and biochemical characterization of an unstudied UGE from the oyster Magallana gigas (MgUGE). Activity tests of recombinantly expressed MgUGE, using HPLC (high-performance liquid chromatography), mass spectrometry, and photometric assays, showed an optimal temperature of 16 °C, and reasonable thermal stability up to 37 °C. No metal ions were required for enzymatic activity. The simple nickel-affinity-purification procedure makes MgUGE a valuable biocatalyst for the synthesis of UDP-galactose from UDP-glucose. The biosynthetic potential of MgUGE was further exemplified in a coupled enzymatic reaction with an oyster-derived β-1,4-galactosyltransferase (MgGalT7), allowing the galactosylation of the model substrate para-nitrophenol xylose (pNP-xylose) using UDP-glucose as the starting material.

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Citations

Jan 1, 2020·Journal of Asian Natural Products Research·Yan YangWei Wang
Sep 15, 2020·Glycoconjugate Journal·Ran CaoJosef Voglmeir
Feb 29, 2020·Asian-Australasian Journal of Animal Sciences·Cong LiJuan J Loor

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

PhyML
LigPlot Plus
Accelrys Discovery Studio Visualizer
MODELLER
MUSCLE
Labplot
BLAST

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