UDP-sugar pyrophosphatase of rat retina: subcellular localization and topography

Journal of Neuroscience Research
J A Martina, H J Maccioni

Abstract

Rat retinal tissue possesses as a developmentally regulated, highly active pyrophosphatase activity that hydrolyzes UDP-GalNAc and UDP-Gal but not CMP-NeuAc (Martina et al.: J Neurochem 62:1274-1280, 1995). We show here that this activity, measured with UDP-[3H]GalNAc as substrate, is associated to the membrane fraction of rat retinal homogenates and, upon subfractionation by isopycnic centrifugation in sucrose density gradients, is concentrated in fractions enriched in light Golgi membranes. We examined also the topographic disposition of the catalytic site of the enzyme in the transverse plane of the membranes by measuring the effect of protease treatment and of added EDTA on its activity. Pronase inhibited 50% of the translocation of UDP-[3H]GalNAc to the lumen of the Golgi vesicles but did not affect the enzyme activity either in the absence or in the presence of detergent. EDTA, a membrane-impermeant molecule, inhibited 90% of the activity of the enzyme but did not affect translocation of UDP-[3H]GalNAc and inhibited only 25% the incorporation of [3H]GalNAc into endogenous glycoconjugates. These results indicate that the translocation of UDP-[3H]GalNAc was not necessary for hydrolysis to occur and strongly suggest that the...Continue Reading

References

Mar 1, 1989·Journal of Neurochemistry·D GravottaH J Maccioni
Jan 1, 1987·Annual Review of Biochemistry·C B Hirschberg, M D Snider
Sep 1, 1972·Archives of Biochemistry and Biophysics·R P DonaldsonC Schnarrenberger
Oct 1, 1981·Journal of Neurochemistry·C A LandaR Caputto
Jul 1, 1980·Journal of Neurochemistry·P PanzettaR Caputto
Mar 1, 1995·Journal of Neurochemistry·J A MartinaH J Maccioni
Sep 1, 1963·Journal of Neurochemistry·L SVENNERHOLM

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Citations

Dec 6, 2007·Journal of Neurochemistry·Hugo J F Maccioni
Mar 7, 1998·The Journal of Biological Chemistry·J A MartinaH J Maccioni
Mar 5, 2011·Journal of Neurochemistry·Hugo J F MaccioniMariana L Ferrari
Mar 5, 1999·Biochimica Et Biophysica Acta·H J MaccioniJ A Martina
Jul 18, 2006·Biochimica Et Biophysica Acta·Gerhild van Echten-Deckert, Thomas Herget

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