UL26-deficient human cytomegalovirus produces virions with hypophosphorylated pp28 tegument protein that is unstable within newly infected cells

Journal of Virology
Joshua MungerThomas Shenk

Abstract

The human cytomegalovirus UL26 open reading frame encodes proteins of 21 and 27 kDa that result from the use of two different in-frame initiation codons. The UL26 protein is a constituent of the virion and thus is delivered to cells upon viral entry. We have characterized a mutant of human cytomegalovirus in which the UL26 open reading frame has been deleted. The UL26 deletion mutant has a profound growth defect, the magnitude of which is dependent on the multiplicity of infection. Two very early defects were discovered. First, even though they were present in normal amounts within mutant virions, the UL99-coded pp28 and UL83-coded pp65 tegument proteins were present in reduced amounts at the earliest times assayed within newly infected cells; second, there was a delay in immediate-early mRNA and protein accumulation. Further analysis revealed that although wild-type levels of the pp28 tegument protein were present in UL26 deletion mutant virions, the protein was hypophosphorylated. We conclude that the UL26 protein influences the normal phosphorylation of at least pp28 in virions and possibly additional tegument proteins. We propose that the hypophosphorylation of tegument proteins causes their destabilization within newly inf...Continue Reading

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Citations

Feb 9, 2012·Proceedings of the National Academy of Sciences of the United States of America·Laura J TerryThomas Shenk
Oct 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Boris BogdanowLüder Wiebusch
Jun 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Robert F Kalejta
Sep 17, 2010·PLoS Pathogens·Martin ZydekLüder Wiebusch
Feb 11, 2014·Viruses·Rebecca Marie SmithJulie Anne Kerry
Jan 21, 2010·Immunopharmacology and Immunotoxicology·Steven FroelichPin Wang
Dec 17, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jesse HwangThomas Shenk
May 29, 2012·Trends in Microbiology·Ritesh Tandon, Edward S Mocarski
Dec 18, 2014·MBio·Emre KoyuncuIleana M Cristea
Dec 14, 2016·The Journal of General Virology·Franz J J RiederChristoph Steininger
Oct 3, 2014·Journal of Virology·Chun MathersJoshua Munger
Feb 15, 2019·Journal of Virology·Christopher M Goodwin, Joshua Munger
Aug 9, 2020·Frontiers in Microbiology·Yu-Qing Wang, Xiang-Yu Zhao
Aug 24, 2018·Viruses·Christopher M GoodwinJoshua Munger
Jan 11, 2019·Nature Communications·Maciej T NogalskiBenjamin D Greenbaum
Feb 12, 2021·Journal of Virology·Morgan Monaghan, Joshua Munger

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