Ulex europaeus agglutinin-I binding to dental primary afferent projections in the spinal trigeminal complex combined with double immunolabeling of substance P and GABA elements using peroxidase and colloidal gold.

Somatosensory & Motor Research
M A MatthewsT V Hernandez

Abstract

Ulex europaeus agglutinin I (UEA-I) is a plant lectin with an affinity for L-fucosyl residues in the chains of lactoseries oligosaccharides associated with medium- and smaller-diameter dorsal root ganglion neurons and their axonal processes. These enter Lissauer's tract and terminate within the superficial laminae of the spinal cord overlapping projections known to have a nociceptive function. This implies that the surface coatings of neuronal membranes may have a relationship with functional modalities. The present investigation further examined this concept by studying a neuronal projection with a nociceptive function to determine whether fucosyl-lactoseries residues were incorporated in its primary afferent terminals. Transganglionic transport of horseradish peroxidase (HRP) following injection into tooth pulp chambers was employed to demonstrate dental pulp terminals in the trigeminal spinal complex, while peroxidase and fluorescent tags were used concomitantly to stain for UEA-I. Double immunolabeling for substance P (SP) and gamma-aminobutyric acid (GABA) using peroxidase and colloidal gold allowed a comparison of the distribution of a known excitatory nociceptive transmitter with that of UEA-I binding in specific subnucl...Continue Reading

References

May 1, 1979·The Journal of Comparative Neurology·R D Broadwell, M W Brightman
Oct 15, 1978·European Journal of Pharmacology·P R Wilson, T L Yaksh
Jan 15, 1978·European Journal of Pharmacology·H K Proudfit, R A Levy
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·L J ReganT M Jessell
Feb 19, 1985·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·T M Jessell, J Dodd
Jan 1, 1988·Somatosensory Research·M A MatthewsT V Hernandez
Oct 1, 1986·The Journal of Cell Biology·L M Greenberger, K H Pfenninger
Oct 21, 1985·Brain Research·L R JohnsonR C Canfield
Aug 1, 1985·Brain Research·D Albe-FessardW D Willis
Apr 29, 1985·Brain Research·J G Broton, J P Rosenfeld
Oct 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·W J StreitS S Spicer
Jan 1, 1973·Annual Review of Biochemistry·H Lis, N Sharon
Dec 1, 1969·The Journal of Comparative Neurology·S Gobel, R Dubner
Jan 1, 1983·Experimental Brain Research·L R JohnsonR C Canfield
Jan 1, 1984·Annual Review of Neuroscience·A I Basbaum, H L Fields
Apr 14, 1982·Biochemical and Biophysical Research Communications·T GioanniniE J Simon
Oct 15, 1983·Experientia·A J CrossJ A Johnson
Sep 10, 1984·The Journal of Comparative Neurology·S N LawsonB H Anderton
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·G M EdelmanC Cohen
Feb 1, 1984·Journal of Neurochemistry·J P ZanettaG Vincendon

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Citations

Feb 22, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·B J Sessle
Aug 24, 2013·PLoS Computational Biology·Karen van EunenBarbara M Bakker
Dec 2, 2017·The Journal of Biological Chemistry·Ashley Solmonson, Ralph J DeBerardinis
Jan 1, 1992·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·N F Capra, D Dessem
Jul 8, 1991·The Journal of Comparative Neurology·J R ClementsA J Beitz

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