Ulinastatin Ameliorates Pulmonary Capillary Endothelial Permeability Induced by Sepsis Through Protection of Tight Junctions via Inhibition of TNF-α and Related Pathways

Frontiers in Pharmacology
Ming FangHua-Dong Wang

Abstract

Background: Increased permeability of pulmonary capillary is a common consequence of sepsis that leads to acute lung injury. In this connection, ulinastatin, a urinary trypsin inhibitor (UTI), is used clinically to mitigate pulmonary edema caused by sepsis. However, the underlying mechanism of UTI in alleviating sepsis-associated pulmonary edema remains to be fully elucidated. As tight junctions (TJs) between the pulmonary microvascular endothelial cells (PMVECs) play a pivotal role in the permeability of pulmonary capillary, this study investigated the effect of UTI on expression of junctional proteins in PMVECs during sepsis. Methods: Male adult Sprague Dawley rats were subjected to cecal ligation and puncture (CLP) and divided into sham, CLP, and UTI+CLP groups. UTI was administered every 8 h for 3 days before CLP. At 48 h after surgery, Evans blue (EB) was administered to evaluate the pulmonary vascular leakage. Histological staining was used for evaluation of lung injury score. Using immunofluorescence staining and Western blot, the expression of junctional proteins (occludin, claudin-5, and ZO-1) in pulmonary endothelia was assessed. In vitro, PMVECs were divided into control, lipopolysaccharide (LPS), and UTI+LPS groups ...Continue Reading

References

Dec 1, 1990·British Journal of Pharmacology·M G SiroisP Sirois
Aug 1, 1980·The Journal of Surgical Research·K A WichtermanI H Chaudry
Nov 21, 2000·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·A D Blann
Sep 25, 2001·Journal of Applied Physiology·S M Dudek, J G Garcia
Sep 17, 2002·Current Opinion in Cell Biology·Shoichiro Tsukita, Mikio Furuse
Feb 10, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Thomas Y MaHamid M Said
Oct 15, 2005·Nature Reviews. Drug Discovery·Jon A BurasMichail Sitkovsky
Nov 1, 2007·Respiratory Research·Emanuela Mazzon, Salvatore Cuzzocrea
Jan 10, 2009·Nature Protocols·Daniel RittirschPeter A Ward
Oct 2, 2009·Journal of the Royal Society, Interface·Maria Iris HermannsC James Kirkpatrick
Feb 13, 2010·Annual Review of Physiology·Yulia Komarova, Asrar B Malik
May 19, 2010·Respiratory Research·Riitta KaarteenahoYlermi Soini
Sep 8, 2010·The New England Journal of Medicine·Warren L Lee, Arthur S Slutsky
Nov 13, 2010·The European Respiratory Journal·L WangS M Dudek
Dec 17, 2010·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·Yi-Zhan CaoMing-Hua Liu
Jan 20, 2011·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Xinying ZhangYulin Wang
Feb 8, 2011·Trends in Microbiology·Lien DejagerClaude Libert
May 3, 2011·American Journal of Respiratory Cell and Molecular Biology·Gustavo Matute-BelloUNKNOWN Acute Lung Injury in Animals Study Group
May 31, 2011·Respiratory Research·Ylermi Soini
Jul 17, 2012·JAMA : the Journal of the American Medical Association·V Marco RanieriArthur S Slutsky
May 21, 2013·Antiviral Research·Susan M ArmstrongWarren L Lee
May 31, 2013·Comprehensive Physiology·Konstantin G BirukovAnna A Birukova
Jun 1, 2013·Comprehensive Physiology·Dorothee Günzel, Michael Fromm
Sep 14, 2013·American Journal of Respiratory Cell and Molecular Biology·Weiguo ChenJeffrey R Jacobson
Mar 22, 2014·Seminars in Immunology·Guadalupe Sabio, Roger J Davis
Aug 1, 2014·Thrombosis and Haemostasis·B Marcos-RamiroJ Millán
Mar 11, 2015·The Journal of Cell Biology·Olga TornavacaMaria S Balda
Feb 26, 2016·Annual Review of Pathology·Cindy Park-Windhol, Patricia A D'Amore
Apr 26, 2016·Biochemical and Biophysical Research Communications·Chunping LiChunting Wang
Mar 2, 2017·Histochemistry and Cell Biology·Andrea PeterStefan Hippenstiel
Apr 5, 2017·Experimental Cell Research·Hannah K CampbellKris A DeMali

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Citations

Aug 3, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jie ZhaoBao-Ping Tian
Nov 12, 2021·FEBS Letters·Sascha Rahn, Christoph Becker-Pauly
Jul 3, 2021·Scientific Reports·Marine AngéChristophe Beauloye

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Methods Mentioned

BETA
bronchoalveolar lavage
ELISA
Assay
Protein Assay
electrophoresis
light microscopy

Software Mentioned

SPSS
Image J

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