Ulipristal acetate induces cell cycle delay and remodeling of extracellular matrix

International Journal of Molecular Medicine
So-Jin ShinEunyoung Ha

Abstract

Uterine leiomyoma is a benign tumor that grows within the muscle tissue of the uterus. Ulipristal acetate (UPA) is a pre‑operative drug used to reduce the size of leiomyoma. The aim of the present study was to examine the in vitro mechanistic details of action of UPA on uterine leiomyomas. Primary cultures of leiomyoma cells were isolated from patient myomectomy specimens and incubated in the presence or absence of UPA at various concentrations. The proliferation, cell viability and doubling time properties of the treated cells were analyzed. In addition, the mRNA and protein expression levels of p21, p27, cyclin E, cyclin‑dependent kinase 2 (CDK2), matrix metalloproteinase (MMP)‑2 and MMP‑9 were examined, as well as the structure of F‑actin in the primary‑cultured leiomyoma cells. The results demonstrated that UPA exerted inhibitory effects on proliferation of primary‑cultured leiomyoma cells. Expression of p21 and p27 was upregulated, while cyclin E and CDK2 were downregulated in UPA‑treated primary‑cultured leiomyoma cells. An increased expression of MMP‑2 was observed in primary‑cultured leiomyoma cells and a leiomyoma tissue sample of a patient with previous history of UPA treatment. Furthermore, a pronounced formation of ...Continue Reading

References

Jan 1, 1986·Annual Review of Biochemistry·T D Pollard, J A Cooper
Oct 1, 1981·Fertility and Sterility·V C Buttram, R C Reiter
Mar 4, 2000·Journal of Cellular Physiology·J Slingerland, M Pagano
Feb 24, 2001·Lancet·E A Stewart
May 15, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·I PetracheJ G Garcia
Jun 27, 2003·Environmental Health Perspectives·Gordon P FlakeDarlene Dixon
Mar 9, 2004·Gynecologic and Obstetric Investigation·Małgorzata WolańskaStefan Jaworski
Oct 12, 2004·Fertility and Sterility·Phyllis C LeppertJames H Segars
Feb 21, 2006·BJOG : an International Journal of Obstetrics and Gynaecology·R A BusfieldN Johnson
May 26, 2006·American Journal of Obstetrics and Gynecology·Michael FlynnEvan Myers
Jul 11, 2008·The Biochemical Journal·Vernonica E Franklin-Tong, Campbell W Gourlay
Aug 2, 2008·Fertility and Sterility·Irina K DimitrovaAndrew P Bradford
Aug 30, 2008·Journal of Korean Medical Science·Sabarish RamachandranChi-Heum Cho
Nov 17, 2009·Cell and Tissue Research·Tim E Cawston, David A Young
Apr 24, 2010·Seminars in Reproductive Medicine·Minnie MalikWilliam H Catherino
Nov 8, 2011·Journal de gynécologie, obstétrique et biologie de la reproduction·S BendifallahH Fernandez
Feb 3, 2012·The New England Journal of Medicine·Jacques DonnezUNKNOWN PEARL II Study Group
May 1, 2012·Journal of Cell Science·Sari TojkanderPekka Lappalainen
Jul 31, 2012·International Journal of Endocrinology·Petr HorakRoman Chmel
Aug 11, 2012·Bioarchitecture·Melissa DesouzaJustine R Stehn
Feb 9, 2013·The Journal of Clinical Endocrinology and Metabolism·Md Soriful IslamPasquapina Ciarmela
Mar 19, 2014·Fertility and Sterility·Jacques DonnezUNKNOWN PEARL III and PEARL III Extension Study Group
Oct 20, 2015·Fertility and Sterility·Jacques DonnezErnest Loumaye
Jan 23, 2016·Best Practice & Research. Clinical Obstetrics & Gynaecology·Banafsheh N KashaniFelice Petraglia

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