Ultra-fast electronic detection of antimicrobial resistance genes using isothermal amplification and Thin Film Transistor sensors

Biosensors & Bioelectronics
Chunxiao HuHywel Morgan

Abstract

A low cost thin-film transistor (TFT) nanoribbon (NR) sensor has been developed for rapid real-time detection of DNA amplification using an isothermal Recombinase Polymerase Amplification (RPA) method. The semiconductor chip measures DNA amplification through a pH change, rather than via fluorescence. The utility of the method was demonstrated by amplifying CTX-M and NDM, two genes that confer bacterial resistance to cephalosporins and carbapenems, respectively. It is shown that this approach provides extremely fast and sensitive detection. It can detect <10 copies of the gene in genomic DNA extracted from E. coli or K. pneumoniae clinical isolates within a few minutes. A differential readout system was developed to minimize the effect of primer-dimer amplification on the assay. The simple device has the potential for low cost, portable and real-time nucleic acid analysis as a Point of Care device.

Citations

Feb 13, 2018·Chemical Communications : Chem Comm·Enrico TenagliaCarlotta Guiducci
Feb 14, 2019·BMC Infectious Diseases·Megan M NelsonJohn R Bracht
Sep 7, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Miyuki Tabata, Yuji Miyahara
Nov 30, 2018·Biosensors & Bioelectronics·Fenxiang ZouChaoyong James Yang
Aug 3, 2020·Biosensors & Bioelectronics·Tianxing JiQiang Zhou
Mar 7, 2021·Antibiotics·Georgia D KaprouMiguel Prieto
Aug 26, 2018·Biosensors & Bioelectronics·Ruxiu LiuA Fatih Sarioglu
May 12, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Abdulrahman K S AyfanAdam D Irwin

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