Ultra-Fast Glyco-Coating of Non-Biological Surfaces

International Journal of Molecular Sciences
Eleanor WilliamsNicolai V Bovin

Abstract

The ability to glycosylate surfaces has medical and diagnostic applications, but there is no technology currently recognized as being able to coat any surface without the need for prior chemical modification of the surface. Recently, a family of constructs called function-spacer-lipids (FSL) has been used to glycosylate cells. Because it is known that lipid-based material can adsorb onto surfaces, we explored the potential and performance of cell-labelling FSL constructs to "glycosylate" non-biological surfaces. Using blood group A antigen as an indicator, the performance of a several variations of FSL constructs to modify a large variety of non-biological surfaces was evaluated. It was found the FSL constructs when optimised could in a few seconds glycosylate almost any non-biological surface including metals, glass, plastics, rubbers and other polymers. Although the FSL glycan coating was non-covalent, and therefore temporary, it was sufficiently robust with appropriate selection of spacer and surface that it could capture anti-glycan antibodies, immobilize cells (via antibody), and withstand incubation in serum and extensive buffer washing, making it suitable for diagnostic and research applications.

References

Sep 30, 2010·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Shalini GautamWilliam J Federspiel
Sep 29, 2011·Glycoconjugate Journal·Tatiana PochechuevaViola Heinzelmann-Schwarz
May 4, 2012·Carbohydrate Research·Elena KorchaginaNicolai Bovin
Jul 22, 2014·Transfusion·Katie BarrStephen Henry
Aug 28, 2014·Virologica Sinica·Natalia A IlyushinaNicolai V Bovin

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Citations

Jul 9, 2016·Biopolymers·Katie BarrNicolai Bovin
Feb 13, 2018·Scientific Reports·Stephen HenryNicolai Bovin
Feb 22, 2017·The International Journal of Artificial Organs·Vladislav SemakViktoria Weber
Jun 6, 2020·ChemistryOpen·Anton ZalyginNicolai Bovin

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Methods Mentioned

BETA
glycosylation
light microscopy

Software Mentioned

Ad
Atri
CMG
DOPE
Atetra

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