Ultra-fast low concentration detection of Candida pathogens utilizing high resolution micropore chips.

Sensors
Rafael MuleroMin Jun Kim

Abstract

Although Candida species are the fourth most common cause of nosocomial blood stream infections in the United States, early diagnostic tools for invasive candidemia are lacking. Due to an increasing rate of candidemia, a new screening system is needed to detect the Candida species in a timely manner. Here we describe a novel method of detection using a solid-state micro-scale pore similar to the operational principles of a Coulter counter. With a steady electrolyte current flowing through the pore, measurements are taken of changes in the current corresponding to the shape of individual yeasts as they translocate or travel through the pore. The direct ultra-fast low concentration electrical addressing of C. albicans has established criteria for distinguishing individual yeast based on their structural properties, which may reduce the currently used methods' complexity for both identification and quantification capabilities in mixed blood samples.

References

Dec 31, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Peter G PappasUNKNOWN Infectious Diseases Society of America
Jan 13, 2004·Journal of Clinical Microbiology·Lynn L HorvathDuane R Hospenthal
Aug 13, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Hilmar WisplinghoffMichael B Edmond
Sep 22, 2005·Lab on a Chip·A Carbonaro, L L Sohn
May 17, 2007·Nano Letters·Meni Wanunu, Amit Meller
Jul 13, 2007·Journal of Clinical Microbiology·Lynn L HorvathDuane R Hospenthal
Feb 12, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·R McMullanR J Hay

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Citations

Apr 17, 2010·Small·William R HesseMinjun Kim
Nov 25, 2014·Journal of Medical Microbiology·Fernando Teles, Jorge Seixas

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Methods Mentioned

BETA
PCR
chip

Software Mentioned

pClamp

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