Ultra-fast stem cell labelling using cationised magnetoferritin

Nanoscale
S Correia CarreiraW Schwarzacher

Abstract

Magnetic cell labelling with superparamagnetic iron oxide nanoparticles (SPIONs) facilitates many important biotechnological applications, such as cell imaging and remote manipulation. However, to achieve adequate cellular loading of SPIONs, long incubation times (24 hours and more) or laborious surface functionalisation are often employed, which can adversely affect cell function. Here, we demonstrate that chemical cationisation of magnetoferritin produces a highly membrane-active nanoparticle that can magnetise human mesenchymal stem cells (hMSCs) using incubation times as short as one minute. Magnetisation persisted for several weeks in culture and provided significant T2* contrast enhancement during magnetic resonance imaging. Exposure to cationised magnetoferritin did not adversely affect the membrane integrity, proliferation and multi-lineage differentiation capacity of hMSCs, which provides the first detailed evidence for the biocompatibility of magnetoferritin. The combination of synthetic ease and flexibility, the rapidity of labelling and absence of cytotoxicity make this novel nanoparticle system an easily accessible and versatile platform for a range of cell-based therapies in regenerative medicine.

References

Jan 1, 1990·Cytometry·S MiltenyiA Radbruch
Dec 15, 1986·Biochemical and Biophysical Research Communications·P A Baeuerle, W B Huttner
Mar 1, 1972·Journal of Ultrastructure Research·D DanonE Skutelsky
Jan 1, 1983·The Journal of Cell Biology·R M SteinmanZ A Cohn
Apr 1, 1994·The Journal of Clinical Investigation·A P HollanderA R Poole
May 1, 1994·Journal of Magnetic Resonance Imaging : JMRI·J W BulteJ A Frank
Jul 1, 1997·Physiological Reviews·S MukherjeeF R Maxfield
May 28, 2003·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·E T AhrensP A Morel
Jul 26, 2003·Radiology·Heike E Daldrup-LinkThomas M Link
Jul 22, 2004·Bioconjugate Chemistry·Catherine M GoodmanVincent M Rotello
Apr 22, 2005·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Charles H CunninghamSteven M Conolly
Nov 26, 2005·European Radiology·Gerhard H SimonHeike E Daldrup-Link
Aug 10, 2006·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Pierre SmirnovOlivier Clément
Dec 21, 2006·Journal of the American Chemical Society·Masaki UchidaTrevor Douglas
Jan 4, 2007·Journal of the American Chemical Society·Michael T KlemTrevor Douglas
May 6, 2008·Biomaterials·Claire Wilhelm, Florence Gazeau
Oct 24, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Yasuhiro IkezoeMasahiko Hara
Oct 29, 2008·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Masaki UchidaTrevor Douglas
May 19, 2009·Molecular Pharmaceutics·Urs O HäfeliDavid Bardenstein
Jun 9, 2009·Journal of Inorganic Biochemistry·Ruimin XingZijian Guo
Jun 16, 2009·Nature Materials·Andre E NelMike Thompson
Jul 16, 2009·Angewandte Chemie·Adam W PerrimanStephen Mann
Aug 12, 2009·Contrast Media & Molecular Imaging·Tobias D HenningHeike E Daldrup-Link
Sep 28, 2010·Methods in Cell Biology·Jan R T van WeeringPaul Verkade
Mar 10, 2011·Journal of the American Chemical Society·Elsa ValeroNatividad Gálvez
Nov 4, 2011·Nano Letters·Robert VáchaDaan Frenkel
Sep 27, 2012·Nanotechnology·Mitsuhiro OkudaWalther Schwarzacher
Nov 13, 2012·International Journal of Nanomedicine·Eleonore Fröhlich

❮ Previous
Next ❯

Citations

Jan 10, 2017·ACS Nano·James P K ArmstrongMolly M Stevens
Jan 23, 2019·Lab on a Chip·James P K ArmstrongMolly M Stevens
Dec 18, 2018·Frontiers in Immunology·Santiago Sánchez-AlonsoArantzazu Alfranca
May 19, 2019·International Journal of Molecular Sciences·Le XueJianfei Sun
Apr 1, 2020·Nanoscale Horizons : the Home for Rapid Reports of Exceptional Significance in Nanoscience and Nanotechnolgy·Ping WangBryan Ronain Smith
May 28, 2016·Experimental Biology and Medicine·James Pk Armstrong, Adam W Perriman
Mar 1, 2021·Life Sciences·Arnab SarkarTapan Kumar Maity

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.