Ultra low dose aerosol challenge with Mycobacterium tuberculosis leads to divergent outcomes in rhesus and cynomolgus macaques

Tuberculosis
S A SharpeM J Dennis

Abstract

Well characterised animal models that can accurately predict efficacy are critical to the development of an improved TB vaccine. The use of high dose challenge for measurement of efficacy in Non-human primate models brings the risk that vaccines with the potential to be efficacious against natural challenge could appear ineffective and thus disregarded. Therefore, there is a need to develop a challenge regimen that is more relevant to natural human infection. This study has established that ultra-low dose infection of macaques via the aerosol route can be reproducibly achieved and provides the first description of the development of TB disease in both rhesus and cynomolgus macaques following exposure to estimated retained doses in the lung of less than 10 CFU of Mycobacterium tuberculosis. CT scanning in vivo and histopathology revealed differences in the progression and burden of disease between the two species. Rhesus macaques exhibited a more progressive disease and cynomolgus macaques showed a reduced disease burden. The ability to deliver reproducible ultra-low dose aerosols to macaques will enable the development of refined models of M. tuberculosis infection for evaluation of the efficacy of novel tuberculosis vaccines t...Continue Reading

References

Sep 5, 1968·Annals of the New York Academy of Sciences·R C Good
Sep 1, 1969·The Journal of Hygiene·H A Druett
Mar 2, 1994·JAMA : the Journal of the American Medical Association·G A ColditzF Mosteller
Dec 13, 2003·Journal of Pharmacological and Toxicological Methods·Justin M Hartings, Chad J Roy
Apr 23, 2004·Journal of Medical Primatology·Bobby J GormusPeter J Didier
Mar 4, 2006·Microbes and Infection·JoAnne L Flynn
Sep 6, 2006·Microbes and Infection·David M LewinsohnDeborah A Lewinsohn
Feb 10, 2009·Vaccine·Masaji OkadaMitsunori Sakatani
Jul 22, 2009·Infection and Immunity·Philana Ling LinJoAnne L Flynn
Jul 14, 2010·The Journal of Infectious Diseases·Angela M GreenJoanne L Flynn
Oct 13, 2010·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Jing ZhangWen-Zhe Ho
Mar 21, 2012·Journal of Medical Primatology·D KaushalA A Lackner
Jun 26, 2013·Antimicrobial Agents and Chemotherapy·Philana Ling LinJoAnne L Flynn
Jun 27, 2013·Journal of Medical Primatology·Morteza RoodgarDavid Glenn Smith
Sep 13, 2014·Cold Spring Harbor Perspectives in Medicine·Charles A Scanga, JoAnne L Flynn
Dec 30, 2014·The Lancet Global Health·Alimuddin ZumlaMatt Oliver
Dec 31, 2014·Infection and Immunity·Juliet C Peña, Wen-Zhe Ho

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Citations

Mar 25, 2016·Expert Review of Vaccines·Helen McShane, Tamara Davenne
Jul 28, 2016·Tuberculosis·Kenneth Barry WalkerLewis K Schrager
Feb 7, 2017·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Pere-Joan Cardona, Ann Williams
Feb 9, 2018·Pathogens·Robert L HunterChinnaswamy Jagannath
Dec 6, 2017·Infection and Immunity·Dominick J LaddyPatricia A Darrah
Aug 8, 2018·The Indian Journal of Medical Research·Amit Kumar Singh, Umesh D Gupta
Jan 16, 2018·Nature Medicine·Scott G HansenLouis J Picker
Aug 14, 2020·PLoS Pathogens·Sharie Keanne C GanchuaJoAnne L Flynn
Jun 30, 2018·Scientific Reports·Pedro M GordalizaJuan José Vaquero
Nov 19, 2019·Frontiers in Immunology·Karin DijkmanFrank A W Verreck
Dec 20, 2019·Scientific Reports·Ana Ortega-GilArrate Muñoz-Barrutia
Jun 28, 2018·Frontiers in Microbiology·Mario A Flores-ValdezAngelo A Izzo
May 31, 2018·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·P M GordalizaJ J Vaquero
Nov 4, 2020·Nature Reviews. Immunology·David S KhouryMiles P Davenport
May 18, 2020·Trends in Microbiology·Riti SharanDeepak Kaushal
Feb 6, 2020·The Lancet Infectious Diseases·Lewis K SchragerOle F Olesen
Apr 3, 2021·Frontiers in Cellular and Infection Microbiology·Hee-Jeong YangLaura E Via
Apr 26, 2021·Molecular Immunology·Julien LemaitrePauline Maisonnasse
Oct 12, 2016·Microbiology Spectrum·Juliet C Peña, Wen-Zhe Ho

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