May 3, 2011

Ultra-performance ion-pairing liquid chromatography with on-line electrospray ion trap mass spectrometry for heparin disaccharide analysis

Analytical Biochemistry
Bo YangRobert J Linhardt

Abstract

A high-resolution method for the separation and analysis of disaccharides prepared from heparin and heparan sulfate (HS) using heparin lyases is described. Ultra-performance liquid chromatography in a reverse-phase ion-pairing mode efficiently separates eight heparin/HS disaccharides. The disaccharides can then be detected and quantified using electrospray ionization mass spectrometry. This method is particularly useful in the analysis of small amounts of biological samples, including cells, tissues, and biological fluids, because it provides high sensitivity without being subject to interference from proteins, peptides, and other sample impurities.

Mentioned in this Paper

Cricetulus
Chinese Hamster
Urine
Heparin
Thymidine
Chromatography
Gas Chromatography-Mass Spectrometry
Distilled water
Chinese Hamster Ovary Cell
Lyase

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