Ultra-Performance Liquid Chromatography-Mass Spectrometry Analysis of Free and Esterified Oxygenated Derivatives from Docosahexaenoic Acid in Rat Brain

Lipids
Charlotte JouvèneMichel Guichardant

Abstract

Docosahexaenoic acid (DHA), a prominent long-chain fatty acid of the omega-3 family, is present at high amount in brain tissues, especially in membrane phospholipids. This polyunsaturated fatty acid is the precursor of various oxygenated lipid mediators involved in diverse physiological and pathophysiological processes. Characterization of DHA-oxygenated metabolites is therefore crucial for better understanding the biological roles of DHA. In this study, we identified and measured, by ultrahigh-performance liquid chromatography coupled with tandem mass spectrometry, a number of oxygenated products derived from DHA in exsanguinated and nonexsanguinated brains. These metabolites were found both in free form and esterified in phospholipids. Interestingly, both (R)- and (S)-monohydroxylated fatty acid stereoisomers were observed free and esterified in phospholipids. Monohydroxylated metabolites were the main derivatives; however, measurable amounts of dihydroxylated products such as protectin DX were detected. Moreover, exsanguination allowed discriminating brain oxygenated metabolites from those generated in blood. These results obtained in healthy rats allowed an overview on the brain oxygenated metabolism of DHA, which deserves ...Continue Reading

References

Oct 30, 1992·Biochimica Et Biophysica Acta·S Yamamoto
Feb 10, 1991·Biochemical and Biophysical Research Communications·H Y KimN Salem
Jan 1, 1988·Advances in Alcohol & Substance Abuse·T ShinguN Salem
Nov 1, 1987·Neurochemical Research·G S HambrechtE F Ellis
Sep 1, 1985·Journal of Neurochemistry·S A AdesuyiE F Ellis
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R L MaasJ A Oates
Jan 1, 1982·Progress in Lipid Research·J Tinoco
Mar 3, 1998·Neurochemical Research·M R PrasadW R Markesbery
Aug 19, 2003·The Journal of Biological Chemistry·Victor L MarcheselliNicolas G Bazan
Sep 30, 2003·Rapid Communications in Mass Spectrometry : RCM·Seon Hwa LeeIan A Blair
Jan 30, 2007·Journal of Neurochemistry·Akhlaq A FarooquiTahira Farooqui
May 24, 2007·The Journal of Biological Chemistry·Benjamin H MaskreyValerie B O'Donnell
Dec 24, 2008·The Journal of Experimental Medicine·Charles N SerhanMatthew Spite
Mar 9, 2010·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Hee-Yong KimYang-Suk Kim
Sep 14, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ping ChenMichel Guichardant
Jul 20, 2011·Chemical Reviews·Charles N Serhan, Nicos A Petasis
Aug 25, 2011·Chemical Reviews·Huiyong YinNed A Porter
Jul 10, 2012·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Sarah K OrrRichard P Bazinet
Oct 16, 2014·Biochimica Et Biophysica Acta·Hartmut KuhnKlaus van Leyen
Nov 13, 2014·Nature Reviews. Neuroscience·Richard P Bazinet, Sophie Layé
Apr 29, 2015·Analytical Chemistry·Hulda S JónasdóttirMartin Giera
Jun 30, 2015·Prostaglandins, Leukotrienes, and Essential Fatty Acids·N M SalemJ R Hibbeln
Dec 10, 2015·Molecular Neurobiology·Mingqin ZhuMarianne Schultzberg

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Citations

Sep 5, 2019·International Journal of Molecular Sciences·Amanda Lo VanNathalie Bernoud-Hubac
Nov 10, 2018·Bioanalysis·Yashpal S ChhonkerDaryl J Murry
Sep 22, 2020·Biochimie·Michel LagardeNathalie Bernoud-Hubac
Oct 11, 2020·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M GuichardantM Lagarde

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