Ultra rapid liquid chromatography as second dimension in a comprehensive two-dimensional method for the screening of pharmaceutical samples in stability and stress studies

Journal of Chromatography. a
A L HuidobroC Barbas

Abstract

Separation orthogonality has been explored with respect to comprehensive two-dimensional liquid chromatography (2D-LC) for different reversed-phase stationary phases. The outcome of this study points out an SB-CN x BEH-C18 combination, used in the first and the second dimension, respectively, as the most orthogonal chromatographic system for the samples assayed. The present investigation reports the employment of an ultra high pressure liquid chromatography system (UPLC) as the second chromatographic dimension, increasing the sensitivity and the speed, completing the whole chromatographic separation in a reasonable time frame. Finally, an off-line 2D-LC method with diode array detection based on the UPLC has been optimized, allowing the separation and minimizing the run time. SB-CN and BEH-C18 were employed as first and second dimension, respectively, with gradient elution applied in each dimension. Alprazolam degraded tablets were studied as a proof of concept of the utility of this type of setups for impurity profiling of complex samples.

References

May 24, 2006·Electrophoresis·Luis SaavedraCoral Barbas
Dec 19, 2006·Journal of Chromatography. a·Djuro Josic, James G Clifton

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Citations

Aug 24, 2013·Journal of Pharmaceutical and Biomedical Analysis·Deepti Jain, Pawan Kumar Basniwal
Mar 27, 2012·Journal of Chromatography. a·Pavel Jandera
Dec 23, 2015·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·René Holm, David P Elder
Apr 29, 2009·Journal of Chromatography. a·Paola DugoLuigi Mondello
Sep 23, 2008·Journal of Chromatography. a·Petr CeslaPavel Jandera
Jan 20, 2009·Journal of Chromatography. a·Jacob N FairchildGeorges Guiochon
Mar 5, 2015·Journal of Separation Science·Danielle N BassanesePaul G Stevenson
Aug 15, 2014·Analytical and Bioanalytical Chemistry·Victoria ElsnerOliver J Schmitz
Jun 29, 2016·Analytical and Bioanalytical Chemistry·Heba Shaaban
Apr 23, 2009·Analytical Chemistry·Krisztián HorváthGeorges Guiochon
May 8, 2009·Analytical Chemistry·R K Gilpin, C S Gilpin
Sep 14, 2012·Analytical Chemistry·Martin GilarAleksander Jaworski

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