Ultra-rapid microwave-stimulated tissue processing with a modified protocol incorporating microwave fixation

Pathology
Zenobia A M Hafajee, Anthony S-Y Leong

Abstract

To develop an ultra-rapid microwave (MW)-stimulated histoprocessing protocol that incorporates MW fixation and produces consistent, high quality sections. A range of fresh autopsy tissues was divided into three groups composed of equal numbers of small and large tissue blocks. Group 1 tissues were fixed for 8 hours in 4% buffered formaldehyde and processed in a conventional tissue processor through a 15-hour cycle. Group 2 tissues were processed in a MW histoprocessor according to the manufacturer's recommended protocol of irradiation in a proprietary reagent before embedding in paraffin. Group 3 tissues were processed with our protocol that incorporated the addition of MW fixation in 4% buffered formaldehyde and MW irradiation in isopropyl alcohol after irradiation in the proprietary reagent. The manufacturer's protocol resulted in 'grey' and 'wet look' artefacts in large tissue blocks. These artefacts did not occur in our protocol which produced sections that were indistinguishable from those obtained with conventional 23-hour processing. Histochemical and immunohistochemical stains were also indistinguishable and the tissue blocks cut very smoothly. The incorporation of an additional step to ensure adequate tissue fixation a...Continue Reading

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Citations

May 26, 2007·Applied Immunohistochemistry & Molecular Morphology : AIMM·Neal S GoldsteinUNKNOWN Members of Ad-Hoc Committee On Immunohistochemistry Standardization
Feb 4, 2010·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Hideki HattaYasuo Takano
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Aug 28, 2021·Journal of Pharmacy & Bioallied Sciences·Pallavi MishraAbikshyeet Panda

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