Ultra-rapid warming yields high survival of mouse oocytes cooled to -196°c in dilutions of a standard vitrification solution.

PloS One
Shinsuke Seki, Peter Mazur

Abstract

Intracellular ice is generally lethal. One way to avoid it is to vitrify cells; that is, to convert cell water to a glass rather than to ice. The belief has been that this requires both the cooling rate and the concentration of glass-inducing solutes be very high. But high solute concentrations can themselves be damaging. However, the findings we report here on the vitrification of mouse oocytes are not in accord with the first belief that cooling needs to be extremely rapid. The important requirement is that the warming rate be extremely high. We subjected mouse oocytes in the vitrification solution EAFS 10/10 to vitrification procedures using a broad range of cooling and warming rates. Morphological survivals exceeded 80% when they were warmed at the highest rate (117,000°C/min) even when the prior cooling rate was as low as 880°C/min. Functional survival was >81% and 54% with the highest warming rate after cooling at 69,000 and 880°C/min, respectively. Our findings are also contrary to the second belief. We show that a high percentage of mouse oocytes survive vitrification in media that contain only half the usual concentration of solutes, provided they are warmed extremely rapidly; that is, >100,000°C/min. Again, the coolin...Continue Reading

References

Oct 27, 1972·Science·D G WhittinghamP Mazur
May 1, 1996·Biology of Reproduction·A MartinoS P Leibo
Aug 26, 1998·Molecular Reproduction and Development·G VajtaH Callesen
Feb 15, 2001·Molecular Reproduction and Development·M Lane, D K Gardner
May 11, 2005·The Journal of Reproduction and Development·Prudencio B PedroMagosaburo Kasai
Jun 25, 2005·Cryobiology·Peter MazurKeisuke Edashige
Dec 22, 2009·Reproductive Biomedicine Online·P VanderzwalmenN H Zech
Feb 2, 2010·Reproductive Biomedicine Online·Ho-Joon LeeThomas L Toth
Feb 18, 2011·Reproductive Biomedicine Online·Ervin MacasBruno Imthurn

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Citations

Jan 31, 2014·Journal of Assisted Reproduction and Genetics·Ri-Cheng ChianYi-Ran Li
Nov 28, 2013·Theriogenology·A Arav
Jul 6, 2014·Biotechnology Journal·Waseem AsgharUtkan Demirci
Apr 14, 2015·Rejuvenation Research·Natasha Vita-More, Daniel Barranco
Apr 23, 2013·Reproductive Biomedicine Online·A PapatheodorouY Prapas
May 25, 2016·Human Fertility : Journal of the British Fertility Society·Shun XiongGuo Ning Huang
Dec 29, 2016·Journal of Assisted Reproduction and Genetics·Miguel GallardoRamón Risco
Jan 25, 2013·Clinical and Experimental Reproductive Medicine·Eun Kyung RyuJin Ho Lim
Dec 4, 2014·Journal of Assisted Reproduction and Genetics·Michael L ReedCharles L Caperton
Jun 21, 2018·Current Protocols in Cell Biology·Jan Huebinger, Markus Grabenbauer
Jul 25, 2018·Nature Communications·Justyna J JaskiewiczSaul Tzipori
May 29, 2016·Journal of Assisted Reproduction and Genetics·Debra A GookDavid H Edgar
Aug 2, 2021·Reproduction in Domestic Animals = Zuchthygiene·Juliana de Souza FernandesLúcia Daniel Machado da Silva

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Methods Mentioned

BETA
transgenic
dissection

Software Mentioned

Cryotops
Graphpad

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