Abstract
Beta amyloid peptide, tau, and phosphorylated tau are well recognized as promising biomarkers for the diagnosis of Alzheimer's disease (AD). In this work, we developed a direct, versatile, and ultrasensitive multiplex assay for the quantification of trace amounts of these protein biomarkers for AD in different types of biological fluids including cerebrospinal fluid, serum, saliva, and urine. The detection assay is based on the immunoreaction between the target proteins and their corresponding pair of antibodies followed by fluorescence labelling with a newly developed indolium-based turn-on fluorophore, namely SIM. SIM was tailor-made as a reporter to provide a high signal-to-noise ratio for the detection assay. An exceptionally low limit of detection down to the femto-molar level was achieved in this assay with minute consumption of the sample. This versatile detection assay is capable of reliably quantifying not only the target proteins simultaneously from a CSF sample in an hour but also trace amounts of protein biomarkers in saliva and urine. This assay has a high potential to serve as a practical tool for the diagnosis of AD.
References
Jun 13, 2003·Journal of Geriatric Psychiatry and Neurology·Linda M ErcoliGary W Small
Nov 11, 2003·The World Journal of Biological Psychiatry : the Official Journal of the World Federation of Societies of Biological Psychiatry·Niels AndreasenKaj Blennow
Apr 11, 2006·Journal of Proteome Research·Erik PorteliusKaj Blennow
Mar 29, 2008·Talanta·Mun'delanji VestergaardEiichi Tamiya
Nov 11, 2008·Journal of Chromatography. a·Romain VerpillotMyriam Taverna
Mar 11, 2009·Archives of Neurology·Tero TapiolaTuula Pirttilä
Jul 23, 2009·JAMA : the Journal of the American Medical Association·Niklas MattssonKaj Blennow
Jul 23, 2009·JAMA : the Journal of the American Medical Association·Ronald C Petersen, John Q Trojanowski
Nov 5, 2010·BMC Neurology·Felix Bermejo-ParejaEva Carro
Nov 16, 2010·Analytical Chemistry·Ning XiaFeimeng Zhou
Aug 4, 2011·Clinics·Luiz Kobuti Ferreira, Geraldo F Busatto
Aug 16, 2011·Journal of Alzheimer's Disease : JAD·Min ShiJing Zhang
Jan 4, 2012·Archives of General Psychiatry·Peder BuchhaveOskar Hansson
May 11, 2012·Journal of Alzheimer's Disease : JAD·Li-San WangChristopher M Clark
Apr 30, 2013·Biosensors & Bioelectronics·Paola GagniMarcella Chiari
Jul 19, 2013·Dementia and Geriatric Cognitive Disorders·Magdalena NutuAnnika Ohrfelt
Oct 12, 2013·PloS One·Jere E MeredithCharles F Albright
Oct 26, 2013·Journal of Alzheimer's Disease : JAD·Takehiro KikoTeruo Miyazawa
Jan 1, 2014·Translational Neuroscience·Mirjana BabićGoran Simić
Mar 15, 2014·Expert Review of Neurotherapeutics·Nathan J WittenbergMoses Rodriguez
May 6, 2014·Alzheimer's & Dementia : the Journal of the Alzheimer's Association·Kaj BlennowHarald Hampel
Aug 20, 2014·Electrophoresis·Raúl González-DomínguezJosé Luis Gómez-Ariza
Aug 21, 2014·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Silvia ContiMirco Vanelli Coralli
Oct 5, 2014·Talanta·Ling YuMaotian Xu
Mar 4, 2015·Analytica Chimica Acta·Yi-Rou WangSu-Hwei Chen
Nov 13, 2015·Analytical and Bioanalytical Chemistry·Pasquale PalladinoGiuseppe Spoto
Feb 7, 2016·Biosensors & Bioelectronics·Ajeet KaushikMadhavan Nair
Aug 30, 2016·Alzheimer's & Dementia : the Journal of the Alzheimer's Association·UNKNOWN Alzheimer's Association
Citations
Jan 9, 2020·Nature Communications·Kayoung KimChan Beum Park
Oct 30, 2019·Critical Reviews in Clinical Laboratory Sciences·Bhavana VeerabhadrappaSylvain Lehmann
Sep 14, 2019·Scientific Reports·Jun Sung ParkMyeong Ok Kim
Mar 23, 2018·Sensors·Dujuan LiMaria Minunni
Mar 22, 2018·Nanoscale·Forough GhasemiMorteza Mahmoudi
Jun 16, 2018·Mass Spectrometry Reviews·Giuseppe Grasso
Nov 12, 2020·Diagnostics·Le Minh Tu PhanSungbo Cho
Jan 8, 2021·International Journal of Molecular Sciences·Simran RastogiSaroj Kumar
Feb 10, 2021·Expert Review of Molecular Diagnostics·Le Minh Tu PhanSungbo Cho
Feb 18, 2021·Analytical Chemistry·Jicheng NiuFei Li
Nov 7, 2020·Analytical Chemistry·Chengjie DuanGenxi Li
Aug 29, 2020·ACS Nano·Yuanyuan MaLehui Xiao
Nov 21, 2018·ACS Sensors·Yan QiZhengping Li
Dec 8, 2021·Analytical Chemistry·Xi WuNa Li
Feb 8, 2022·Mikrochimica Acta·Yibiao LiuTailin Xu