Ultra-sensitive fully automated immunoassay for detection of propanil in aqueous samples: steps of progress toward sub-nanogram per liter detection

Analytical and Bioanalytical Chemistry
Jens TschmelakGuenter Gauglitz

Abstract

The widely-used pesticide propanil is a selective post-emergent general-use acetanilide herbicide registered for control of broadleaf and grass weeds in rice, small grain, and turf. Because broad application and quite heavy use of this herbicide lead to contaminated sites and, consequently, contaminated water, immunoanalytical methods with very low limits of detection (LOD) and low limits of quantification (LOQ) are becoming increasingly important for environmental analysis and, especially, for monitoring drinking-water quality. Environmental monitoring of pesticides, hormones, endocrine-disrupting chemicals, and antibiotics in aqueous samples (e.g. surface, ground, waste, or drinking water) with quite difficult matrices places large demands on chemical analysis. Biosensors have suitable characteristics such as efficiency in enabling very fast, sensitive, and cost-effective detection. Here we describe the steps of progress toward sub-nanogram per liter detection of propanil with a fully automated immunoassay. In contrast with common analytical methods such as GC-MS or HPLC-MS the biosensor used requires no sample pre-treatment and pre-concentration. The basis of our sensitive assay is an antibody with a high affinity constant t...Continue Reading

Citations

Apr 28, 2005·Biosensors & Bioelectronics·Chris Rowe TaittFrances S Ligler
Jun 30, 2006·Analytical and Bioanalytical Chemistry·Sara Rodriguez-MozazDamià Barceló
Jun 22, 2005·Expert Review of Proteomics·Martin Dufva, Claus B V Christensen
May 28, 2008·Analytical and Bioanalytical Chemistry·Michael Seidel, Reinhard Niessner
Jul 19, 2005·Analytical and Bioanalytical Chemistry·Jens TschmelakGuenter Gauglitz
Jan 31, 2008·Chemical Reviews·Sergey M Borisov, Otto S Wolfbeis
Nov 16, 2005·Analytical Chemistry·Madalina TudoracheJenny Emnéus

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.