PMID: 44586Jan 1, 1979

Ultracytochemical characterization of non-specific acid phosphatase activities in Saccharomyces cerevisiae

Zeitschrift für allgemeine Mikrobiologie
K J Böhm, E Unger

Abstract

Glutaraldehyde prefixation causes a considerable inactivation of the acid phosphatase of yeast protoplasts in dependence on the duration of aldehyde influence. Lead ions necessary for ultracytochemical demonstration effect a still stronger inhibition of enzymatic activity. Prefixation, however, protects the enzyme from further inhibition by lead. At pH 4.4 in intact cells acid phosphatase activities are mainly localized in the periplasmic space and in vesicles fused with the plasma membrane. The cell wall and cytoplasm usually remain free of reaction products. On the cell surface activities are found in form of globular lead deposits. At pH 5.2 and 6.3 the periplasmic activity appears decreased compared to that at lower pH values and the intracellular activity is increased. The plasma membrane of protoplasts is completely free of precipitates. The intracellular activity sites of protoplasts (cisternae of endoplasmic reticulum and/or Golgi-like system, small vesicles, central vacuole, nuclear envelope) are the same as for intact cells. The occurrence of at least two forms of acid phosphatase in S. cerevisiae id deduced.

References

Sep 1, 1975·Journal of Bacteriology·H J Van RijnP Boer
Aug 1, 1977·Journal of Bacteriology·W A LinnemansP F Elbers
Jan 1, 1978·Zeitschrift für allgemeine Mikrobiologie·E StreiblováJ Kybal
Sep 1, 1976·Chemico-biological Interactions·E Sabbioni, E Marafante
Aug 1, 1971·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S J OrlowskiS S Herman
Jan 1, 1971·Zeitschrift für allgemeine Mikrobiologie·R May
Oct 1, 1969·The Journal of Cell Biology·C H DamskyA Claude
Nov 15, 1966·Biochimica Et Biophysica Acta·P Boer, E P STEYN-PARVE
Feb 12, 1963·Biochimica Et Biophysica Acta·W L MCLELLAN, J O LAMPEN
Jan 18, 1963·Biochemical and Biophysical Research Communications·C F HEREDIAA SOLS
Mar 12, 1963·Biochimica Et Biophysica Acta·G J TONINO, E P STEYN-PARVE

Citations

Jan 1, 1980·Molecular & General Genetics : MGG·P Lange, P E Hansche

Related Concepts

Acid Phosphatase
Plasma Membrane
Protoplasm
Endoplasmic Reticulum
Sekumatic
Cytochemistry
Hydrogen-Ion Concentration
Lead
Protoplasts
Saccharomyces cerevisiae

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