Ultradian Secretion of Growth Hormone in Mice: Linking Physiology With Changes in Synapse Parameters Using Super-Resolution Microscopy.

Frontiers in Neural Circuits
Klaudia BednarzThomas Stroh

Abstract

Neuroendocrine circuits are orchestrated by the pituitary gland in response to hypothalamic hormone-releasing and inhibiting factors to generate an ultradian and/or circadian rhythm of hormone secretion. However, mechanisms that govern this rhythmicity are not fully understood. It has been shown that synaptic transmission in the rodent hypothalamus undergoes cyclical changes in parallel with rhythmic hormone secretion and a growing body of evidence suggests that rapid rewiring of hypothalamic neurons may be the source of these changes. For decades, structural synaptic studies have been utilizing electron microscopy, which provides the resolution suitable for visualizing synapses. However, the small field of view, limited specificity and manual analysis susceptible to bias fuel the search for a more quantitative approach. Here, we apply the fluorescence super-resolution microscopy approach direct Stochastic Optical Reconstruction Microscopy (dSTORM) to quantify and structurally characterize excitatory and inhibitory synapses that contact growth hormone-releasing-hormone (GHRH) neurons during peak and trough values of growth hormone (GH) concentration in mice. This approach relies on a three-color immunofluorescence staining of G...Continue Reading

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Citations

Sep 3, 2021·Endocrinology and Metabolism·Bo Hye KimObin Kwon

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Methods Mentioned

BETA
transmission electron microscopy
fluorescence
super-resolution microscopy
ELISA
light scattering
transgenic
fluorescence microscopy

Software Mentioned

R
Excel
SRX
Graph Pad Prism
DBSCAN
DBSCAN ( Spatial Clustering of Applications
d STORM
RLA
STORM
Vutara SRX

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