Ultrafast Electron Transfer in Complexes of Doxorubicin with Human Telomeric G-Quadruplexes and GC Duplexes Probed by Femtosecond Fluorescence Spectroscopy

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
Pascale Changenet-BarretIlse Manet

Abstract

Doxorubicin (DOX) is a natural anthracycline widely used in chemotherapy; its combined application as a chemotherapeutic and photodynamic agent has been recently proposed. In this context, understanding the photoinduced properties of DOX complexes with nucleic acids is crucial. Herein, the study of photoinduced electron transfer in DOX-DNA complexes by femtosecond fluorescence spectroscopy is reported. The behaviour of complexes with two model DNA structures, a G-quadruplex (G4) formed by the human telomeric sequence (Tel21) and a d(GC) duplex, is compared. The DOX affinity for these two sequences is similar. Although both 1:1 and 2:1 stoichiometries have been reported for DOX-G4 complexes, only 1:1 complexes form with the duplex. The steady-state absorption indicates a strong binding interaction with the duplex due to drug intercalation between the GC base pairs. In contrast, the interaction of DOX with Tel21 is much weaker and arises from drug binding on the G4 external faces at two independent binding sites. As observed for DOX-d(GC) complexes, fluorescence of the drug in the first binding site of Tel21 exhibits decays within a few picoseconds following a biphasic pattern; this is attributed to the existence of two drug conf...Continue Reading

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Citations

Jul 8, 2016·Organic & Biomolecular Chemistry·Erica SalvatiIlse Manet
Jun 21, 2017·Science Advances·Jialong JieHongmei Su
Mar 27, 2019·Physical Chemistry Chemical Physics : PCCP·Valentin MaffeisAlessandro Venturini
Jun 22, 2021·Chemistry : a European Journal·Francesco ManoliIlse Manet
Jan 30, 2018·The Journal of Physical Chemistry. B·Sneha Paul, Anunay Samanta

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