Ultrafast Excited-State Deactivation of 8-Hydroxy-2'-deoxyguanosine Studied by Femtosecond Fluorescence Spectroscopy and Quantum-Chemical Calculations

The Journal of Physical Chemistry. a
Pascale Changenet-BarretDimitra Markovitsi

Abstract

The fluorescence properties of the 8-hydroxy-2'-deoxyguanosine (8-oxo-dG) in aqueous solution at pH 6.5 are studied by steady-state spectroscopy and femtosecond fluorescence up-conversion and compared with those of 2'-deoxyguanine (dG) and 2'-deoxyguanine monophosphate (dGMP). The steady-state fluorescence spectrum of 8-oxo-dG is composed of a broad band that peaks at 356 nm and extends over the entire visible spectral region, and its fluorescence quantum yield is twice that of dG/dGMP. After excitation at 267 nm, the initial fluorescence anisotropy at all wavelengths is lower than 0.1, giving evidence of an ultrafast internal conversion (<100 fs) between the two lowest excited ππ* states (Lb and La). The fluorescence decays of 8-oxo-dG are biexponential with an average lifetime of 0.7 ± 0.1 ps, which is about two times longer than that of dGMP. In contrast with dGMP, only a moderate dynamical shift (∼1400 vs 10,000 cm(-1)) of the fluorescence spectra of 8-oxo-dG is observed on the time scale of a few picoseconds without modification of the spectral shape. PCM/TD-DFT calculations, employing either the PBE0 or the M052X functionals, provide absorption spectra in good agreement with the experimental one and show that the deactiva...Continue Reading

References

Nov 1, 1989·Chemical Research in Toxicology·S J CulpF E Evans
Dec 3, 2003·Journal of the American Chemical Society·Tatiana GiorgiGiovanni Gottarelli
Jul 9, 2004·Biochimica Et Biophysica Acta·Ivo H M van StokkumRienk van Grondelle
Aug 12, 2005·Chemical Reviews·Jacopo TomasiRoberto Cammi
May 6, 2006·The Journal of Chemical Physics·Hui Chen, Shuhua Li
Feb 6, 2007·The Journal of Physical Chemistry. a·Christel M Marian
Jan 25, 2008·Journal of the American Chemical Society·Luis Serrano-AndrésAntonio Carlos Borin
Aug 1, 2008·Accounts of Chemical Research·Jean CadetJean-Luc Ravanat
Oct 31, 2008·The Journal of Physical Chemistry. a·Shohei YamazakiAndrzej L Sobolewski
Jan 9, 2009·Journal of the American Chemical Society·Tal Z MarkusCynthia J Burrows
Feb 5, 2009·The Journal of Physical Chemistry. a·Namrata JayanthMrinalini Puranik
Apr 28, 2009·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Zhenggang LanWalter Thiel
Sep 29, 2009·The Journal of Physical Chemistry. B·Roberto ImprotaFabrizio Santoro
Nov 27, 2009·Accounts of Chemical Research·Sriram KanvahUzi Landman
Jan 21, 2010·The Journal of Physical Chemistry. a·Francois-Alexandre MiannayDimitra Markovitsi
Sep 1, 2011·Journal of the American Chemical Society·Khiem Van Nguyen, Cynthia J Burrows
May 10, 2012·Physical Chemistry Chemical Physics : PCCP·Berit HeggenWalter Thiel
Mar 30, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Chopen Chan-Wut ChengWai-Ming Kwok
Apr 26, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Thomas GustavssonRoberto Improta
Nov 13, 2013·The Journal of Physical Chemistry. a·Yuyuan ZhangBern Kohler
Nov 23, 2013·Topics in Current Chemistry·Angelo GiussaniManuela Merchán
Mar 22, 2014·Topics in Current Chemistry·Roberto Improta, Vincenzo Barone
Jul 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Yuyuan ZhangBern Kohler
Oct 3, 2014·Topics in Current Chemistry·Sebastian MaiLeticia González

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Citations

Dec 10, 2015·Physical Chemistry Chemical Physics : PCCP·Deniz Tuna, Wolfgang Domcke
Jun 9, 2015·The Journal of Physical Chemistry. B·Zhen LuSpiridoula Matsika
Jan 19, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiuxiu WuWolfgang Domcke
Mar 21, 2020·Scientific Reports·Jean Philippe RenaultMarie Géléoc
Nov 26, 2021·Methods and Applications in Fluorescence·Muhammad Farooq Saleem KhanZhou Xu

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