PMID: 374570Jan 1, 1979Paper

Ultrafast laser scanner microscope

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
R V ShackP H Bartels

Abstract

Advances in monolayer deposition of cervical cells have removed one of the last serious obstacles to the design of high-resolution automated diagnostic assessment systems. In this article, we describe the design considerations for a system that is capable of acquiring, within 60 sec, a 0.5 micron digitized image of a 4 cm2 area on a standard glass slide. The most feasible approach is found to be a system using a rotating polygon to sweep the focused spot from a laser across a 2-mm scan line while the slide is uniformly translated perpendicular to the scan direction the use of laser sources (a helium-neon laser at 632 nm and a krypton ion laser at 568 and/or 476 nm) as compared to the incoherent light sources used in conventional microscope systems alleviates many of the optical design problems and provides the proper wavelengths needed for recognition of Papanicolaou stained cells. We also find that focus control of the scanning spot should be achievable using a technique involving a holographic grating. Other relevant considerations such as sample heating problems, multiphoton absorption by the sample, detector signal-to-noise ratios, laser amplitude noise control, and the digitization and buffering of the data stream are also...Continue Reading

Citations

Apr 27, 2004·Experimental Eye Research·C B CourvilleS D Klyce
Nov 1, 1987·Quarterly Reviews of Biophysics·Z Kam
Sep 29, 2000·Cornea·S D Klyce
Feb 1, 1982·IEEE Transactions on Bio-medical Engineering·R L ShoemakerD Vukobratovich
Feb 1, 1982·IEEE Transactions on Bio-medical Engineering·I T YoungR P Bishop
Mar 1, 1988·Cytometry·D Burger, R Gershman
Jan 25, 2012·Journal of Biophotonics·Ivana K CecicCalum MacAulay
Dec 1, 1985·Journal of Microscopy·E W HansenM F Riley
Mar 15, 1981·Applied Optics·D Kessler, R V Shack

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