Ultrafast laser-scanning time-stretch imaging at visible wavelengths

Light, Science & Applications
Jianglai WuKevin K Tsia

Abstract

Optical time-stretch imaging enables the continuous capture of non-repetitive events in real time at a line-scan rate of tens of MHz-a distinct advantage for the ultrafast dynamics monitoring and high-throughput screening that are widely needed in biological microscopy. However, its potential is limited by the technical challenge of achieving significant pulse stretching (that is, high temporal dispersion) and low optical loss, which are the critical factors influencing imaging quality, in the visible spectrum demanded in many of these applications. We present a new pulse-stretching technique, termed free-space angular-chirp-enhanced delay (FACED), with three distinguishing features absent in the prevailing dispersive-fiber-based implementations: (1) it generates substantial, reconfigurable temporal dispersion in free space (>1 ns nm-1) with low intrinsic loss (<6 dB) at visible wavelengths; (2) its wavelength-invariant pulse-stretching operation introduces a new paradigm in time-stretch imaging, which can now be implemented both with and without spectral encoding; and (3) pulse stretching in FACED inherently provides an ultrafast all-optical laser-beam scanning mechanism at a line-scan rate of tens of MHz. Using FACED, we demo...Continue Reading

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Citations

Jul 7, 2018·Nature Protocols·Cheng LeiKeisuke Goda
Jul 7, 2019·Nature Protocols·Akihiro IsozakiKeisuke Goda
May 23, 2020·FEBS Letters·Anna E Eastman, Shangqin Guo
Jul 10, 2020·Lab on a Chip·Akihiro IsozakiKeisuke Goda
Jul 11, 2018·Journal of Biophotonics·Bo DaiXu Wang
Apr 30, 2020·Nature Communications·Joseph C JingLihong V Wang
Nov 24, 2017·Lab on a Chip·Massimiliano M VillonePietro Ferraro
Mar 2, 2019·Optica·Jinyang Liang, Lihong V Wang
May 1, 2020·Light, Science & Applications·Pablo Loza-Alvarez
May 15, 2020·Optics Express·Yicai LiuZhongmin Yang
May 31, 2019·Physical Review Letters·Yu LuLidai Wang
Dec 26, 2018·Expert Opinion on Drug Discovery·Andrea K PomerantzThomas Krucker
Mar 16, 2019·Optics Letters·Xianglei LiuJinyang Liang
Aug 2, 2018·Optics Letters·Cihang KongKenneth K Y Wong
Apr 15, 2020·Optics Letters·Yueyun WengCheng Lei
Jan 16, 2019·Applied Optics·Michael E DurstEmma Moskovitz
Nov 28, 2020·Nature Communications·Jiang Lan FanNa Ji
Oct 30, 2020·Optics Express·Yueyun WengCheng Lei
Apr 21, 2021·Light, Science & Applications·Pablo Loza-Alvarez
Apr 26, 2021·Trends in Biotechnology·Kelvin C M LeeKevin K Tsia
Aug 4, 2021·Nature Protocols·Queenie T K LaiKevin K Tsia
Sep 22, 2021·Nature Communications·Shobana V StassenKevin K Tsia
May 15, 2017··Kenneth K. Y. WongJianglai Wu
Feb 28, 2017··Ata MahjoubfarJiahao Lin

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Methods Mentioned

BETA
optical microscopy
chip
biopsy
chips
density-gradient centrifugation
fluorescence
flow cytometry
fluorescence imaging

Software Mentioned

FACED
MATLAB

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