Ultrahigh-mass mass spectrometry of single biomolecules and bioparticles

Annual Review of Analytical Chemistry
Huan-Cheng Chang

Abstract

Since the advent of soft ionization methods, mass spectrometry (MS) has found widespread application in the life sciences. Mass is now known to be a critical parameter for characterization of biomolecules and their complexes; it is also a useful parameter to characterize bioparticles such as viruses and cells. However, because of the genetic diversity of these entities, it is necessary to measure their masses individually and to obtain the corresponding mean masses and mass distributions. Here, I review recent technological developments that enable mass measurement of ultrahigh-mass biomolecules and bioparticles at the single-ion level. Some representative examples include cryodetection time-of-flight MS of single-megadalton protein ions, Millikan-type mass measurements of single viruses in a cylindrical ion trap, and charge-detection quadrupole ion trap MS of single whole cells. I also discuss the promises and challenges of these new technologies in real-world applications.

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Citations

Aug 3, 2010·Journal of the American Society for Mass Spectrometry·Harry J SterlingEvan R Williams
May 25, 2011·Proceedings of the National Academy of Sciences of the United States of America·Weihua GuanMark A Reed
Jul 20, 2010·Annual Review of Analytical Chemistry·Mélanie AbonnencHubert H Girault
Dec 3, 2013·The Review of Scientific Instruments·Brandon L BarneyDaniel E Austin
Apr 25, 2012·Annual Review of Analytical Chemistry·Christie G EnkeDavid W Koppenaal
Feb 14, 2016·Mass Spectrometry Reviews·David Z Keifer, Martin F Jarrold
May 12, 2016·Analytical Chemistry·Ning ZhangZongxiu Nie
Mar 11, 2015·Nature Communications·Eric SageSébastien Hentz
Aug 23, 2019·The Analyst·Abdil ÖzdemirChung-Hsuan Chen
Mar 29, 2013·Analytical Chemistry·Caiqiao XiongZongxiu Nie
Nov 22, 2017·Analytical Chemistry·Abdil OzdemirChung-Hsuan Chen
Oct 15, 2019·Analytical Chemistry·Caiqiao XiongZongxiu Nie
Jul 19, 2019·Analytical Chemistry·Xi HuangZongxiu Nie

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Methods Mentioned

BETA
flow cytometry
light scattering

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