Ultrahigh-resolution, high-speed spectral domain optical coherence phase microscopy

Optics Letters
Rehman AnsariGereon Hüttmann

Abstract

We present an ultrahigh-resolution, high-speed spectral domain optical coherence phase microscopy (SD-OCPM) system that combines submicrometer transverse spatial resolution and subnanometer optical path length sensitivity, with an acquisition speed of over 217,000 voxels/s. The proposed SD-OCPM system overcomes two significant drawbacks of traditional common-path interferometers-limited transverse spatial resolution and suboptimal detection sensitivity-while maintaining phase stability that is comparable with common-path interferometer setups. The transverse and axial spatial resolution of the setup is measured to be 0.6 and 1.9 μm, respectively, with a phase sensitivity of 0.0027 rad (corresponds to optical path length sensitivity of 110 pm). High-speed acquisition allows for phase-sensitive 4D imaging of biological samples with subcellular resolution.

References

Jul 21, 2004·Optics Letters·Christopher G RylanderAshley J Welch
Jun 11, 2005·Optics Letters·Michael A ChomaJoseph A Izatt
May 6, 2006·Journal of Biomedical Optics·Michael A ChomaJoseph A Izatt
Aug 14, 2009·Biosensors & Bioelectronics·Chulmin JooJohannes F de Boer
Apr 22, 2011·Applied Optics·Andra St QuintinMarinko V Sarunic
Dec 12, 2012·Journal of Biomedical Optics·Itay ShockNatan T Shaked
Mar 5, 2013·Optics Letters·Frank HeldermanMattijs de Groot

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Citations

Apr 16, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Asylkhan RakhymzhanRaluca A Niesner
Mar 20, 2018·Biomedical Optics Express·Hinnerk Schulz-HildebrandtGereon Hüttmann
Mar 18, 2015·Biomedical Optics Express·Ruibo ShangYizheng Zhu

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