Ultraperformance ion-pair liquid chromatography coupled to electrospray time-of-flight mass spectrometry for compositional profiling and quantification of heparin and heparan sulfate

Analytical Chemistry
Albert K KorirCynthia K Larive

Abstract

Heparin and heparan sulfate (HS) are important pharmaceutical targets because they bind a large number of proteins, including growth factors and cytokines, mediating many biological processes. Because of their biological significance and complexity, there is a need for development of rapid and sensitive analytical techniques for the characterization and compositional analysis of heparin and HS at the disaccharide level, as well as for the structure elucidation of larger glycosaminoglycan (GAG) sequences important for protein binding. In this work, we present a rapid method for analysis of disaccharide composition using reversed-phase ion-pairing ultraperformance liquid chromatography coupled with electrospray time-of-flight mass spectrometry ((RPIP)-UPLC-MS). Heparin disaccharide standards were eluted in less than 5 min. The method was used to determine the constituents of GAGs from unfractionated heparin/HS from various bovine and porcine tissues, and the results were compared with literature values.

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Citations

Oct 9, 2008·Analytical and Bioanalytical Chemistry·Albert K Korir, Cynthia K Larive
Sep 21, 2010·Analytical and Bioanalytical Chemistry·Bo YangRobert J Linhardt
Sep 4, 2010·Analytical and Bioanalytical Chemistry·John F K LimtiacoCynthia K Larive
Nov 21, 2013·Analytical and Bioanalytical Chemistry·Anna AlekseevaAnnamaria Naggi
Apr 13, 2011·Applied Microbiology and Biotechnology·Zhenyu WangRobert J Linhardt
May 14, 2010·Journal of the American Society for Mass Spectrometry·Alan G GonçalvesMiguel D Noseda
Dec 21, 2010·Journal of Medicinal Chemistry·Zhongping XiaoRobert J Linhardt
May 11, 2010·Omics : a Journal of Integrative Biology·Mellisa LyRobert J Linhardt
Apr 8, 2011·Annual Review of Analytical Chemistry·Christopher J JonesCynthia K Larive
May 13, 2014·Analytical and Bioanalytical Chemistry·Ebru UcakturkRobert J Linhardt
Sep 8, 2009·Analytical Biochemistry·Ponnusamy Babu, Balagurunathan Kuberan
May 29, 2012·Journal of Pharmaceutical and Biomedical Analysis·Bo WangDavid A Keire
Jun 21, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jia Yan DuZheng Wei
Mar 21, 2017·Journal of Cellular Biochemistry·Abdolreza Ardeshirylajimi
Oct 18, 2008·Journal of Separation Science·Justin G StrohLisa M Nogle
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Jan 1, 2013·Biomolecules·Jun-Ichi FurukawaYasuro Shinohara
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Sep 23, 2008·Carbohydrate Research·Stacie L EldridgeCynthia K Larive
Oct 6, 2021·Mass Spectrometry Reviews·Andreas ZappeKevin Pagel
Aug 10, 2011·Carbohydrate Research·John F K LimtiacoCynthia K Larive

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