Ultrasensitive detection of telomerase activity in a single cell using stem-loop primer-mediated exponential amplification (SPEA) with near zero nonspecific signal

Chemical Science
Honghong WangZhengping Li

Abstract

Telomerase is a crucial biomarker for cancers. Its reliable and sensitive detection, particularly in a single cell, has great significance for the early diagnosis of cancers, studies on tumor progression and anticancer therapy, which remain a challenge, due to nonspecific amplification. Herein, we developed a novel stem-loop primer-mediated exponential amplification (SPEA) strategy, which can specifically and efficiently amplify the telomerase-elongated telomere repeat unit with near zero nonspecific signal. The SPEA-based assay can accurately detect telomerase activity in the crude lysate of a single cell and is suited for detecting the cellular heterogeneity arising from cell-to-cell variations.

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Citations

Apr 22, 2020·Organic & Biomolecular Chemistry·Pengbo ZhangZhengping Li
Jul 1, 2017·Nanoscale Horizons : the Home for Rapid Reports of Exceptional Significance in Nanoscience and Nanotechnolgy·Suyan QiuWei-Chuan Shih
Aug 3, 2018·Chemical Communications : Chem Comm·Chen-Chen LiChun-Yang Zhang
Sep 14, 2018·Biosensors & Bioelectronics·Lixu WangHuanghao Yang

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

SPEA

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