Ultrasensitive detection of Vibrio cholerae O1 using microcantilever-based biosensor with dynamic force microscopy

Biosensors & Bioelectronics
Usa SungkanakAdisorn Tuantranont

Abstract

This work presents the first demonstration of a cantilever based cholerae sensor. Dynamic force microscopy within atomic force microscope (AFM) is applied to measure the cantilever's resonance frequency shift due to mass of cell bound on microcantilever surface. The Vibrio cholerae O1, a food and waterborne pathogen that caused cholera disease in human, is a target bacterium cell of interest. Commercial gold-coated AFM microcantilevers are immobilized with monoclonal antibody (anti-V. cholerae O1) by self-assembled monolayer method. V. cholerae O1 detection experiment is then conducted in concentrations ranging from 1×10(3) to 1×10(7) CFU/ml. The microcantilever-based sensor has a detection limit of ∼1×10(3) CFU/ml and a mass sensitivity, Δm/ΔF, of ∼146.5 pg/Hz, which is at least two orders of magnitude lower than other reported techniques and sufficient for V. cholerae detection in food products without pre-enrichment steps. In addition, V. cholerae O1 antigen-antibody binding on microcanilever is confirmed by scanning electron microscopy. The results demonstrate that the new biosensor is promising for high sensitivity, uncomplicated and rapid detection of V. cholerae O1.

Citations

Jul 19, 2012·World Journal of Microbiology & Biotechnology·Kim-Fatt LowYean-Yean Chan
Oct 20, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Phuong Dinh Tam, Cao Xuan Thang
Aug 29, 2013·Journal of Analytical Methods in Chemistry·Shuchen HsiehChun-Hsin Wu
Jul 13, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mireia Hoyos-NoguésCarlos Mas-Moruno
Jul 2, 2014·Clinical Microbiology Reviews·Asif AhmedPaul A Millner
Mar 10, 2020·Research : a Science Partner Journal·Xiaodi SuXian Jun Loh
Aug 28, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Pratima R SolankiB D Malhotra
Aug 1, 2020·Enzyme and Microbial Technology·Aviru Kumar BasuShantanu Bhattacharya
Jul 24, 2021·Biotechnology and Applied Biochemistry·Sebnem SeherlerIlke Anac Sakir

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthrax Vaccines

Three different types of anthrax vaccines are available; a live-attenuated, an alum-precipitated cell-free filtrate and a protein recombinant vaccine. The effectiveness between the three is uncertain, but the live-attenuated have shown to reduce the risk of anthrax with low adverse events. Here is the latest research on anthrax vaccines.

Anthrax

Anthrax toxin, comprising protective antigen, lethal factor, and oedema factor, is the major virulence factor of Bacillus anthracis, an agent that causes high mortality in humans and animals. Here is the latest research on Anthrax.

Anthrax Vaccines (ASM)

Three different types of anthrax vaccines are available; a live-attenuated, an alum-precipitated cell-free filtrate and a protein recombinant vaccine. The effectiveness between the three is uncertain, but the live-attenuated have shown to reduce the risk of anthrax with low adverse events. Here is the latest research on anthrax vaccines.