Ultrasensitive determination of receptor tyrosine kinase with a label-free electrochemical immunosensor using graphene quantum dots-modified screen-printed electrodes

Analytica Chimica Acta
Fariba MollarasouliKarim Asadpour-Zeynali

Abstract

A new label-free electrochemical immunosensor is constructed for the selective and sensitive determination of the clinically relevant biomarker receptor tyrosine kinase (AXL) in human serum. The disposable immunosensing platform is prepared by immobilization of the specific anti-AXL antibody onto amine functionalized graphene quantum dots (fGQDs)-modified screen-printed carbon electrodes (SPCEs). The affinity reactions were monitored by measuring the decrease in the differential pulse voltammetric (DPV) response of the redox probe Fe(CN)63-/4-. All the experimental variables involved in the preparation of the modified electrodes and in the immunosensor performance were optimized. The as prepared immunosensor exhibits an improved analytical performance with respect to other electrochemical immunosensors reported so far, with a wider range of linearity and a lower detection limit, 0.5 pg mL-1, which is more than one hundred thousand times lower than the established cut-off value for heart failure (HF) diagnosis in serum (71 ng mL-1). The developed immunosensor was successfully applied to the determination of the endogenous content of AXL in serum of HF patients without any matrix effect observed after just a sample dilution.

Citations

May 21, 2019·Critical Reviews in Analytical Chemistry·Goksu OzcelikaySibel A Ozkan
Dec 28, 2019·Materials·Bhargav D Mansuriya, Zeynep Altintas
Sep 19, 2019·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Wenxia WangXiaoli Lan
Apr 24, 2019·Nanomaterials·Susana CampuzanoJosé M Pingarrón
Feb 23, 2020·Sensors·Bhargav D Mansuriya, Zeynep Altintas
Sep 11, 2020·Biosensors·Lasangi DhanapalaJames F Rusling
Jul 22, 2019·Biosensors·Fariba MollarasouliSibel A Ozkan
Apr 8, 2020·Biosensors·Beatriz Pérez-FernándezAlfredo de la Escosura- Muñiz
Sep 22, 2020·Critical Reviews in Analytical Chemistry·Nurgul K BakirhanSibel A Ozkan
May 4, 2021·Advanced Materials·Peng WangYuHuang Wang

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