Ultrasensitive DNA biosensor for hepatitis B virus detection based on tin-doped WO3/In2O3 heterojunction nanowire photoelectrode under laser amplification.

Analytical and Bioanalytical Chemistry
Mohsen Shariati, Mahdi Sadeghi

Abstract

The fabrication of a highly sensitive DNA biosensor based on tin-doped WO3/In2O3 nanowires as heterojunction photoelectrode for detection of hepatitis B virus is reported. The tin-doped WO3/In2O3 nanowires were fabricated via a physical vapor deposition mechanism and were nearly 50 nm in width. The single-strand DNA probe was covalently immobilized on the nanowire surface. The biosensor could detect the hybridization of complementary DNA in a label-free approach at very low concentrations. The biodetection processes were conducted through reduction-oxidation reactions in the electrochemical impedance spectral measurements. The electrochemical impedance responses were biased under laser amplification to achieve the detection limit of 1 fM. The fabricated biosensor could detect DNA concentrations from 0.1 pM to 10 μM linearly in the calibration plot. Due to laser amplification, more charged carriers were released and they interacted with DNA on the electrode surface. The efficiency of the charge transfer parameter was enhanced by a photogeneration process, and the electron-hole recombination rate could intensively increase biosensor sensitivity, selectivity, and distinguishability. The stability of the nanowire biosensor under la...Continue Reading

References

May 15, 2002·Journal of Virological Methods·D ParaskevisA Hatzakis
Mar 17, 2004·Journal of Pharmaceutical and Biomedical Analysis·Ourania E TsitsilonisPeggy Lymberi
Dec 3, 2004·Frontiers in Bioscience : a Journal and Virtual Library·Huangxian Ju, Hongtao Zhao
Oct 27, 2006·The Analyst·Pavel TakmakovSergei Smirnov
Dec 15, 2006·Journal of the American Chemical Society·Yuri L BunimovichJames R Heath
Feb 26, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Ting CaiZhonghua Meng
Mar 22, 2008·Biosensors & Bioelectronics·Guo-Jun ZhangN Balasubramanian
Dec 22, 2009·Biosensors & Bioelectronics·Bongkeun KangKyung-Hwa Yoo
Jan 6, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Shashishekar P AdigaRoger J Narayan
Nov 3, 2010·Analytica Chimica Acta·Rebecca L CaygillPaul A Millner
Mar 11, 2011·Trends in Biotechnology·Pieter Stroeve, Nazar Ileri
Aug 14, 2012·ACS Nano·Alfredo de la Escosura-Muñiz, Arben Merkoçi
Jun 24, 2017·Bioelectrochemistry·Laleh Enayati Ahangar, Masoud A Mehrgardi
Jan 29, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Babak NegahdariAli Asghar Saeedi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.