Ultrasensitive electrochemical immunosensor based on horseradish peroxidase (HRP)-loaded silica-poly(acrylic acid) brushes for protein biomarker detection

Biosensors & Bioelectronics
Yan ZhaoFengli Qu

Abstract

We report an ultrasensitive electrochemical immunosensor designed for the detection of protein biomarkers using horseradish peroxidase (HRP)-loaded silica-poly(acrylic acid) brushes (SiO2-SPAABs) as labels. HRP could be efficiently and stably accommodated in the three-dimensional architecture of the SiO2-SPAABs and the SiO2-SPAABs-HRP exhibited high catalytic performance towards o-phenylenediamine (OPD) oxidation in the presence of H2O2, which resulted in significant differential pulse voltammetric (DPV) response change and color change. Using human IgG (HIgG) as a model analyte, a sandwich-type immunosensor was constructed. In particular, graphene oxide (GO) and SiO2-SPAABs-HRP were used to immobilize capture antibody (Ab1) and bind a layer of detection antibody (Ab2), respectively. The current biosensor exhibited a good linear response of HIgG from 100pg/mL to 100μg/mL with a detection limit of 50pg/mL (S/N=5). The sensitivity was 6.70-fold higher than the conventional enzyme-linked immunosorbent assays. The immunosensor results were validated through the detection of HIgG in serum samples.

Citations

May 8, 2016·Biosensors & Bioelectronics·Haimeng PeiFengli Qu
May 4, 2016·Applied Microbiology and Biotechnology·Airis Maria Araújo MeloCarlúcio R Alves
Feb 28, 2019·Biotechnology Journal·Dani PermanaNoriho Kamiya
Dec 23, 2020·Nanoscale·Indra Memdi KhorisEnoch Y Park
Mar 30, 2018·ACS Applied Materials & Interfaces·Sangjin OhJaebeom Lee
Oct 24, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Sang Hun ChunSoong Ho Um
Oct 18, 2019·ACS Nano·Carmen C Mayorga-MartinezMartin Pumera
Jan 21, 2017·Analytical Chemistry·Wei WenYuehe Lin

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