Ultrasensitive fluorescent proteins for imaging neuronal activity

Nature
Tsai-Wen ChenDouglas S Kim

Abstract

Fluorescent calcium sensors are widely used to image neural activity. Using structure-based mutagenesis and neuron-based screening, we developed a family of ultrasensitive protein calcium sensors (GCaMP6) that outperformed other sensors in cultured neurons and in zebrafish, flies and mice in vivo. In layer 2/3 pyramidal neurons of the mouse visual cortex, GCaMP6 reliably detected single action potentials in neuronal somata and orientation-tuned synaptic calcium transients in individual dendritic spines. The orientation tuning of structurally persistent spines was largely stable over timescales of weeks. Orientation tuning averaged across spine populations predicted the tuning of their parent cell. Although the somata of GABAergic neurons showed little orientation tuning, their dendrites included highly tuned dendritic segments (5-40-µm long). GCaMP6 sensors thus provide new windows into the organization and dynamics of neural circuits over multiple spatial and temporal scales.

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Jun 20, 2018·Journal of Biophotonics·Michael A TaylorEthan K Scott
Aug 14, 2018·ELife·Ben DeverettSamuel S-H Wang
Sep 12, 2017·ELife·Sebastian A Vasquez-LopezJohannes C Dahmen
Jan 26, 2018·ELife·Juan Ramón Martínez-FrançoisGary Yellen
Jan 30, 2018·IEEE Transactions on Biomedical Circuits and Systems·Diederik Paul MoeysTobi Delbruck
Feb 15, 2017·Journal of Biomedical Optics·Olivier ThouveninClaude Boccara
Sep 1, 2018·Cerebral Cortex·Kjell HeuserRune Enger
Sep 20, 2018·Diabetes, Obesity & Metabolism·Jing W HughesDavid W Piston
Aug 21, 2018·ELife·Romain FranconvilleVivek Jayaraman
Sep 13, 2018·PLoS Biology·Alessandro D UboldiChristopher J Tonkin
Sep 14, 2018·Proceedings of the National Academy of Sciences of the United States of America·Niels NiethardJan Born
Aug 4, 2018·Anesthesiology·Andrew E Hudson
Nov 22, 2013·Circulation Research·Wei ShangHeping Cheng
Feb 1, 2014·Circulation Research·Yan-Ting Zhao, Héctor H Valdivia
May 9, 2014·Circulation Research·Lars KaestnerPeter Lipp
Sep 29, 2018·PLoS Computational Biology·Marcus A TriplettGeoffrey J Goodhill
Jul 19, 2014·Circulation Research·Xiang Luo, Joseph A Hill
Aug 16, 2017·Proceedings of the National Academy of Sciences of the United States of America·Giancarlo N BruniJoel M Kralj
Aug 31, 2018·ELife·Xiaowei HouStephen Barstow Long
Sep 26, 2018·The European Journal of Neuroscience·Carole MorelMing-Hu Han
Feb 20, 2016·PLoS Computational Biology·Vahid RahmatiStefan J Kiebel
Aug 5, 2017·PLoS Computational Biology·Johannes FriedrichLiam Paninski
Dec 20, 2017·Proceedings of the National Academy of Sciences of the United States of America·James A StrotherMichael B Reiser
Dec 14, 2016·Science Advances·Seung Woo LeeShelley I Fried
Oct 13, 2016·Nature Communications·Awani UpadhyayWendy Hanna-Rose
Oct 4, 2016·Nature Communications·Olena RiabininaChristopher J Potter
Oct 28, 2016·Nature Communications·Jie YangCarl Hirschie Johnson
Feb 26, 2016·Nature Communications·Julia CoxYang Dan
Aug 3, 2016·Nature Communications·Dario L RingachJoshua T Trachtenberg
Jun 14, 2016·Nature Communications·Timothy H MurphyJeff M LeDue
Nov 14, 2018·Nature Methods·Madhuvanthi KannanVincent A Pieribone
Dec 1, 2018·PLoS Genetics·Elizabeth Medina-CastellanosAlfredo Herrera-Estrella
Dec 18, 2018·Current Protocols in Cytometry·Jared M EllerXiaolu A Cambronne
Nov 24, 2018·ELife·Michael KruminMatteo Carandini
Dec 27, 2018·American Journal of Primatology·Jung Eun Park, Afonso C Silva
Aug 18, 2018·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Joseph A FantuzzoZhiping P Pang
Jan 8, 2019·Cerebral Cortex·Michael J SiniscalchiAlex C Kwan
Jan 15, 2019·Investigative Ophthalmology & Visual Science·Stephanie N BlandfordSpring R M Farrell
Nov 18, 2018·The Journal of Experimental Biology·Mehmet F KeleşMark A Frye
Jan 19, 2019·Science·Gregory CorderGrégory Scherrer
Jan 22, 2019·Nature Methods·Yong QianRobert E Campbell
Jan 26, 2018·Nature Communications·Franz WeberYang Dan
Feb 1, 2018·Scientific Reports·Yusaku OhtaKazuki Horikawa
Jan 31, 2019·The New England Journal of Medicine·Bradford B Lowell
Feb 25, 2018·Nature Neuroscience·Dayu Lin
Feb 7, 2019·ELife·Anzhelika KoldaevaIzumi Fukunaga
Jun 24, 2018·Chembiochem : a European Journal of Chemical Biology·Yong QianRobert E Campbell