Ultrasensitive magnetic field-assisted surface plasmon resonance immunoassay for human cardiac troponin I

Biosensors & Bioelectronics
Qiong WuDaqian Song

Abstract

An ultrasensitive surface plasmon resonance (SPR) immunoassay was developed for the specific detection of human cardiac troponin I (cTnI), a principle diagnostic marker for myocardial damage. The thin gold film evaporated on a glass slate, which was employed as the SPR sensing film, was modified by hollow gold nanoparticles (HGNPs) and polydopamine (PDA) sequentially, and then was immobilized with antibodies for specific recognition of target analyte. Electronic coupling of the surface plasmon waves originating from the HGNPs and the gold film leads to the remarkable amplification of SPR response. The PDA film modified on the gold film via self-polymerization of dopamine (DA) facilitates the direct immobilization of capture antibodies (cAb). To separate and enrich the target analyte, PDA-wrapped magnetic multi-walled carbon nanotubes (MMWCNTs-PDA) were conjugated with detection antibodies (dAb) and used as the extracting agent for the magnetic extraction of cTnI in sample. Large surface area of MMWCNTs-PDA ensures its loading capacity for dAb, as well as its extraction efficiency for cTnI. By serving as the "vehicles" for fast delivering the concentrated analyte to the SPR sensing surface, MMWCNTs-PDA-dAb also overcomes the dis...Continue Reading

Citations

Dec 19, 2018·Biosensors·Ewa Gorodkiewicz, Zenon Lukaszewski
Aug 3, 2019·Journal of Clinical Laboratory Analysis·Jiye WangBinjie Wang
Oct 31, 2019·The Analyst·Elba MaurizLaura M Lechuga
Jan 30, 2020·Nanomaterials·Mingfei PanShuo Wang
Apr 3, 2020·Micromachines·Muqsit Pirzada, Zeynep Altintas
Dec 8, 2019·Sensors·Muqsit Pirzada, Zeynep Altintas
Jan 4, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Sanam DolatiAhmad Mobed
Jul 24, 2021·Biotechnology and Applied Biochemistry·Sebnem SeherlerIlke Anac Sakir
Dec 16, 2020·ACS Biomaterials Science & Engineering·Suliman KhanMojtaba Falahati

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.