Ultrasensitive multi-analyte electrochemical immunoassay based on GNR-modified heated screen-printed carbon electrodes and PS@PDA-metal labels for rapid detection of MMP-9 and IL-6

Biosensors & Bioelectronics
Jian-Jun ShiJun-Jie Zhu

Abstract

An ultrasensitive electrochemical immunoassay was developed for rapid detection of interleukin-6 (IL-6) and matrix metallopeptidase-9 (MMP-9); the method utilized PS@PDA-metal nanocomposites based on graphene nanoribbon (GNR)-modified heated screen-printed carbon electrode (HSPCE). Because of the good hydrophilicity and low toxicity, GNRs were used to immobilize antibodies (Ab) and amplify the electrochemical signal. PS@PDA-metal was used to label antibodies and generate a strong electrochemical signal in acetic buffer. A sandwich strategy was adopted to achieve simultaneous detection of MMP-9 and IL-6 based on HSPCE without cross-talk between adjacent electrodes in the range of 10(-5) to 10(3) ng mL(-1) with detection limits of 5 fg mL(-1) and 0.1 pg mL(-1) (S/N=3), respectively. The proposed method showed wide detection range, low detection limit, acceptable stability and good reproducibility. Satisfactory results were also obtained in the practical samples, thus showing this is a promising technique for simultaneous clinical detection of biocomponent proteins.

References

Mar 9, 2000·Journal of Pharmaceutical and Biomedical Analysis·J W FindlayR R Bowsher
Nov 3, 2005·Journal of the American Chemical Society·Savka I StoevaChad A Mirkin
Oct 20, 2007·Science·Haeshin LeePhillip B Messersmith
Apr 4, 2008·Nature·Samir M HanashVitor M Faca
Apr 17, 2009·Nature·Dmitry V KosynkinJames M Tour
Jul 7, 2010·Cell·Laurent Kappeler, Michael J Meaney
Apr 13, 2011·Journal of Colloid and Interface Science·Wencai WangLiqun Zhang
Oct 21, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Jinhong JiangYouyi Xu
Apr 9, 2013·Food Chemistry·Chi-Yu ZhangRong-Rong Lu

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Citations

Jul 19, 2016·Chemical Reviews·Mahmoud LabibShana O Kelley
Apr 8, 2017·Sensors·Benoît Piro, Steeve Reisberg
Apr 8, 2016·Chemical Society Reviews·Adriano AmbrosiMartin Pumera
Nov 22, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zhen LeiZhenxin Wang
Apr 26, 2021·Biosensors & Bioelectronics·Asha P JohnsonK Pramod
Apr 29, 2014·International Journal of Biological Macromolecules·E S Abdel-Halim, Salem S Al-Deyab
Jun 7, 2021·Analytical and Bioanalytical Chemistry·Ramón Lorenzo-GómezMaría Jesús Lobo-Castañón

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