Ultrasensitive peptide-based multiplexed electrochemical biosensor for the simultaneous detection of Listeria monocytogenes and Staphylococcus aureus.

Mikrochimica Acta
Shimaa Eissa, Mohammed Zourob

Abstract

A novel electrochemical biosensor is reported for simultaneous detection of two of the most common food-borne pathogens: Listeria monocytogenes and Staphylococcus aureus. The biosensor is composed of an array of gold nanoparticles-modified screen-printed carbon electrodes on which magnetic nanoparticles coupled to specific peptides were immobilized via streptavidin-biotin interaction. Taking advantage of the proteolytic activities of the protease enzymes produced from the two bacteria on the specific peptides, the detection was achieved in 1 min. The detection was realized by measuring the percentage increase of the square wave voltammetric peak current at 0.1 V versus a Ag/AgCl reference electrode in ferro/ferricyanide redox couple after incubation with the bacteria protease. The integration of the specificity of the bacterial enzymes towards their peptide substrates with the sensitivity of the electrochemical detection on the sensor array allows the rapid, sensitive and selective quantification of the two bacteria. Outstanding sensitivities were achieved using this biosensor array platform with limit of detection of 9 CFU mL-1 for Listeria monocytogenes and 3 CFU mL-1 for Staphylococcus aureus. The multiplexing capability and...Continue Reading

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Citations

Mar 5, 2021·Biosensors & Bioelectronics·Veronika VanovaVojtech Adam
Apr 6, 2021·Advances in Colloid and Interface Science·Ashis TripathyFilipe Samuel Silva
May 28, 2021·Mikrochimica Acta·Shimaa EissaMohammed Zourob
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Antonella Curulli
Aug 27, 2021·Biosensors·Patrick Severin SfraganoIlaria Palchetti
Sep 30, 2021·Analytical Methods : Advancing Methods and Applications·Qingcao LiQiaoping Wu
Jan 29, 2022·ACS Applied Materials & Interfaces·Antonio MinopoliRaffaele Velotta
Feb 9, 2022·Mikrochimica Acta·Masoud Negahdary, Lúcio Angnes

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